2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE CAS:2816-24-2

Enzyme substrate: ONPG inowanzoshandiswa se substrate ye beta-galactosidase, enzyme inoita hydrolyzes ONPG kuburitsa yero-ruvara komboni (o-nitrophenol) inogona kuonekwa nyore spectrophotometrically.Iyi enzymatic reaction inogona kushandiswa kuyera chiitiko che beta-galactosidase, ichiita ONPG chishandiso chakakosha muzvidzidzo zve enzymology.

Molecular biology assays: ONPG inoshandiswa se substrate mune akasiyana ma molecular biology assays, kunyanya mu beta-galactosidase reporter gene assays.Mune aya maassays, iyo ONPG-yakavakirwa substrate inoshandiswa kuyera chiitiko chemutori wenhau gene, iro rinowanzo kudzorwa neimwe inosimudzira inoteedzana yekufarira.Iyo beta-galactosidase chiitiko, inoratidzwa nekushandurwa kwemavara inogadzirwa paONPG hydrolysis, inopa ruzivo rwakakosha nezve chiitiko chekusimudzira.

Kuongororwa kweGene kutaura: ONPG inoshandiswawo mukuongorora kwemazwi ejeni.Nekubatanidza iyo inosimudzira kutevedzana kwekufarira kune beta-galactosidase gene, vaongorori vanogona kuyera iyo beta-galactosidase chiitiko vachishandisa ONPG senge substrate.Chiyero chebeta-galactosidase chiitiko chinoratidza simba uye basa rekusimudzira, zvichibvumira kuongororwa kwemazinga ekutaura kwemajini.

Diagnostic applications: ONPG inogona kushandiswa mukuongorora maapplication, kunyanya mukuona kwepathogenic microorganisms.Mabhakitiriya akasiyana, akadai seEscherichia coli uye mamwe marudzi eShigella neSalmonella, anogadzira beta-galactosidase inogona kutsemura ONPG.Iyi hydrolysis reaction inoburitsa inooneka shanduko yeruvara, iyo inogona kushandiswa kuona kuvepo kweaya mabhakitiriya mumakiriniki samples.