4-Nitrophenyl-beta-D-glucopyranoside CAS:2492-87-7

Enzyme chiitiko assay: Iyo hydrolysis ye4-Nitrophenyl-beta-D-glucopyranoside nema enzymes se β-glucosidase inogona kushandiswa kuyera enzyme basa.Kuburitswa kwe4-nitrophenol kunogona kuverengerwa uchishandisa spectrophotometry, zvichibvumira kutsunga kwe enzyme kinetics uye inhibition zvidzidzo.

Gene kutaura uye mutori wenhau assays: 4-Nitrophenyl-beta-D-glucopyranoside inogona kusanganiswa nemamwe ma tag ma molecular anosanganiswa mumaprotein efusion.Chiitiko che enzyme cheprotein yefusion chinogona kuverengerwa zviri nyore nekuyera kuburitswa kwe4-nitrophenol, ichipa manzwisisiro mukutaura kwegene uye kupindirana kweprotein-protein.

Kuwanikwa kwezvinodhaka uye kutarisisa kwepamusoro-soro: Iyo hydrolysis ye4-Nitrophenyl-beta-D-glucopyranoside inogona kushandiswa seyekuita chiitiko chekuongorora kuwanikwa kwezvinodhaka.Inobvumira kuongororwa nekukurumidza kwemaraibhurari makuru emukomboni kuti aone zvingangoita enzyme inhibitors kana activators.

Kuongororwa kwekuongorora: Zvimwe zvirwere zvine chekuita neshanduko mune zvimwe zviitiko zve enzyme.Nekushandisa iyo hydrolysis ye4-Nitrophenyl-beta-D-glucopyranoside nema enzymes chaiwo seyedzo yekuongorora, zvirwere izvi zvinogona kuonekwa kana kuongororwa.Izvi zvinosanganisira mamiriro ane chekuita neiyo lysosomal kuchengetedza kusagadzikana uye hepatobiliary zvirwere.