IPTG CAS:367-93-1 Mutengo wemugadziri
Isopropyl β-D-1-thiogalactopyranoside (IPTG) is a synthetic analogi ye lactose iyo inowanzoshandiswa mukutsvagisa molecular biology uye biotechnology application.IPTG inonyanya kushandiswa kukurudzira kutaura kwemajini muhurongwa hwemabhakitiriya, uko inoshanda senge molecular trigger kutanga kunyorwa kwemajini anotangwa.
Kana yawedzerwa kune yekukura svikiro, IPTG inotorwa nebhakitiriya uye inogona kusunga kune lac repressor protein, ichidzivirira kubva pakuvhara basa re lac operon.Iyo lac operon iboka remajini anobatanidzwa mu lactose metabolism, uye kana puroteni inodzvinyirira yabviswa, majini anoratidzirwa.
IPTG inowanzo shandiswa pamwe chete nelacUV5 mutant mutsigiri, inova vhezheni inoshanda yelac inosimudzira.Nekubatanidza IPTG induction neiyi mutant inokurudzira, vaongorori vanogona kuwana yakakwira nhanho dzekutaura kwejeni.Izvi zvinobvumira kugadzirwa kwehuwandu hwakawanda hweprotein yekucheneswa kana mamwe maapplication ezasi.
Pamusoro pekutaura kwemajini, IPTG inowanzoshandiswa mublue/white screening assays.Muhunyanzvi uhu, iyo lacZ gene inowanzosanganiswa kune gene rekufarira, uye mabhakitiriya anonyatso kuratidza iyi fusion gene anoburitsa inoshanda β-galactosidase enzyme.Apo IPTG inowedzerwa pamwe chete nechromogenic substrate yakadai se X-gal, mabhakitiriya anoratidza gene gene anoshanduka kuva bhuruu nekuda kwebasa re β-galactosidase.Izvi zvinobvumira kucherechedzwa uye kusarudzwa kwezvekudzokororwa kwemhando dzakabudirira kubatanidza gene rekufarira.
Induction of gene expression: IPTG inowanzoshandiswa kukurudzira kutaura kwemajini anotangwa mumabhakitiriya masisitimu.Inotevedzera yakasikwa inducer lactose uye inosunga kune lac repressor protein, ichidzivirira kubva pakuvhara lac operon.Izvi zvinobvumira kunyorwa uye kutaura kwemajini anodiwa.
Protein kutaura uye kucheneswa: IPTG induction inowanzoshandiswa kubudisa mapuroteni akawanda anobatanidzwa nokuda kwezvinangwa zvakasiyana-siyana, zvakadai sezvidzidzo zvebiochemical, kugadzirwa kwekurapa, kana kuongorora maitiro.Nekushandisa akakodzera mavekita ekutaura uye IPTG induction, vaongorori vanogona kuwana yakakwira nhanho yechinangwa chekugadzirwa kweprotein mumabhakitiriya anotambira.
Bhuruu / chena kuongorora: IPTG inowanzoshandiswa pamwe chete nelacZ gene uye chromogenic substrate, senge X-gal, yeblue/white screening assays.Iro lacZ gene rinowanzo kusanganiswa kune gene rekufarira, uye mabhakitiriya anonyatso kuratidza iyi fusion gene anoburitsa inoshanda β-galactosidase enzyme.Kana IPTG uye iyo chromogenic substrate yawedzerwa, madhindindi anodzokorodza anoratidza fusion gene anoshanduka bhuruu, zvichibvumira kuzivikanwa uye kusarudza nyore.
Kudzidza nezve gene regulation: IPTG induction inowanzoshandiswa mukutsvagisa kudzidza kudzorwa kwemajini nemaoperen, kunyanya iyo lac operon.Nekugadzirisa kutariswa kweIPTG uye nekutarisa kutaura kwelac operon zvikamu, vaongorori vanogona kuongorora maitirwo ekugadzirisa magene uye basa rezvakasiyana zvinhu kana shanduko.
Gene kutaura masisitimu: IPTG chinhu chakakosha mune akati wandei magene ekutaura masisitimu, senge T7 inosimudzira-yakavakirwa masisitimu.Mune aya masisitimu, iyo lac inosimudzira inowanzoshandiswa kutyaira kutaura kweT7 RNA polymerase, iyo, inozonyora zvinonangwa majini pasi pekutonga kweT7 kukurudzira kutevedzana.IPTG inoshandiswa kukwevera kutaura kweT7 RNA polymerase, zvichitungamira ku activation yechinangwa chejini kutaura.
| Composition | C9H18O5S |
| Assay | 99% |
| Chitarisiko | Upfu chena |
| CAS Nha. | 367-93-1 |
| Packing | Zvidiki uye zvakawanda |
| Sherufu Hupenyu | 2 years |
| Storage | Chengetedza munzvimbo inotonhorera uye yakaoma |
| Certification | ISO. |
