Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside CAS:24404-53-3

Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside inowanzoshandiswa se substrate ye enzyme β-galactosidase.Kana β-galactosidase ichishanda pairi, musanganiswa unoitwa hydrolyzed, zvichiita kuti pave nekugadzirwa kwep-nitrophenol kana o-nitrophenol, iyo ine ruvara rweyero inogona kuonekwa nyore nyore.Izvi zvinoita kuti zvive zvakakosha se substrate yeassays inoyera β-galactosidase chiitiko.

Imwe yakajairika application yePhenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside iri mune reporter gene assays.Mune aya assays, jini rekufarira rinosanganiswa kumutori wenhau, senge beta-galactosidase gene.Basa remutori wenhau gene rinobva raongororwa nekuyera β-galactosidase basa uchishandisa Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside se substrate.Izvi zvinobvumira vaongorori kuti vadzidze gene expression uye regulation.

Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside inogonawo kushandiswa kuvharidzira kune inhibitors kana activators ye β-galactosidase.Nekuyedza makomisheni akasiyana kana vakwikwidzi vezvinodhaka, vaongorori vanogona kuona mamorekuru anogadzirisa chiitiko cheiyo enzyme, inogona kuve nekurapa kwekushandisa mune zvimwe zvirwere kana mamiriro.

Mukuwedzera, iyi musanganiswa inogona kushandiswa mukucheneswa kweprotein.β-galactosidase inowanzosanganiswa kune mapuroteni ekufarira kuti afambise kuona kwavo nekucheneswa.Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside inogona kushandiswa se substrate kuvhara uye kuchenesa mapuroteni aya ekubatanidza nekuona basa re β-galactosidase.