X-GAL CAS:7240-90-6 Mutengo wemugadziri

Kuchinja Kwemavara: X-Gal kazhinji haina ruvara asi, pa hydrolysis ne β-galactosidase, inoshanduka kuita bhuruu.Uku kushanduka kwemavara kunobvumira kuonekwa kwekuona uye quantification yebasa re β-galactosidase.

LacZ Gene Detection: X-Gal inoshandiswa kuona maseru kana majini anovaka anoratidza lacZ gene.LacZ inowanzoshandiswa semutori wenhau gene mune molecular biology kuongorora gene kutaura kana kudzidza kusimudzira chiitiko.

Colony Screening: X-Gal inowanzoshandiswa mumabhakitiriya koloni yekuongorora assays.LacZ-inoratidza mabhakitiriya makoroni anoita sebhuruu kana akura paagar ine X-Gal, zvichiita kuti zvizivikanwe zviri nyore uye kusarudzwa kwelacZ-positive colonies.

Gene Fusion Analysis: X-Gal inoshandiswawo mukuedza kwemajini fusion.Kana jini rinonangwa rakabatana nelacZ gene, kusviba kweX-Gal kunogona kuratidza chimiro chekutaura kweprotein yefusion mukati mesero kana tishu.

Protein Localization: X-Gal tsvina inogona kushandiswa kuongorora subcellular protein yenzvimbo.Nekusanganisa puroteni yekufarira kune lacZ gene, chiitiko che β-galactosidase chinogona kuratidza panowanikwa puroteni mukati mesero.

X-Gal Analogues: Mafomu akagadziridzwa eX-Gal, akadai seBluo-Gal kana Red-Gal, akagadzirwa kuti abvumire mamwe marongero ekuvandudza mavara.Aya analogues anogonesa kusiyanisa pakati pelacZ-positive uye lacZ-negative masero kana matishu achishandisa mavara akasiyana.