2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE CAS:2816-24-2

2-Nitrophenyl-beta-D-glucopyranoside ikemikari inoumbwa nemorekuru yeglucopyranoside yakabatana neboka renitrophenyl. Inowanzoshandiswa sesubstrate mukuyedza ma enzymes kuona uye kuyera mashandiro ema enzymes akadai se beta-glucosidase. Boka renitrophenyl rinogona kupatsanurwa ne enzyme, zvichikonzera kuburitswa kwechinhu chine ruvara rweyero chinogona kuyerwa ne spectrophotometric. Iyi kemikari inonyanya kubatsira mukudzidza kinetics ye enzyme uye kuongorora kwakanyanya kwe enzyme inhibitors kana activators. Inoshandiswawo mukutsvagisa kwe biochemical kuferefeta metabolism ye carbohydrate uye se substrate chaiyo ye glycosidic-linkage.

Odha kana Kumbira Mutengo

Ruzivo rweChigadzirwa

Matagi eChigadzirwa

Kushandiswa uye Mhedzisiro

Substrate yeenzyme: ONPG inowanzoshandiswa sesubstrate ye beta-galactosidase, enzyme inonyungudutsa ONPG kuti igadzire chinhu chine ruvara rweyero (o-nitrophenol) chinogona kuonekwa zviri nyore kuburikidza ne spectrophotometric. Maitiro aya e enzyme anogona kushandiswa kuyera mashandiro e beta-galactosidase, zvichiita kuti ONPG ive chishandiso chakakosha muzvidzidzo zve enzymology.

Kuyedzwa kweMolecular biology: ONPG inoshandiswa sechikamu chepasi mukuyedzwa kwakasiyana-siyana kweMolecular biology, kunyanya mukuyedzwa kweBeta-galactosidase reporter gene. Mukuyedzwa uku, chikamu cheONPG chinoshandiswa kuyera mashandiro eGene reporter, izvo zvinowanzo tungamirirwa nepromoter sequence chaiyo yefariro. Basa reBeta-galactosidase, rinoonekwa nekuchinja kweruvara kunogadzirwa paONPG hydrolysis, rinopa ruzivo rwakakosha nezvebasa repromoter.

Kuongorora matauriro emajini: ONPG inoshandiswawo mukuongorora matauriro emajini. Nekubatanidza kutevedzana kwepromoter kune gene re beta-galactosidase, vaongorori vanogona kuyera chiitiko che beta-galactosidase vachishandisa ONPG se substrate. Huremu hwechiitiko che beta-galactosidase hunoratidza simba nebasa repromoter, zvichibvumira kuongororwa kwemazinga ekutaura kwemajini.

Mashandisirwo ekuongorora: ONPG inogona kushandiswa mukuongorora, kunyanya mukuona tupukanana tunokonzera zvirwere. Mabhakitiriya akasiyana-siyana, akadai seEscherichia coli nedzimwe mhando dzeShigella neSalmonella, anogadzira beta-galactosidase iyo inogona kupatsanura ONPG. Kuchinja uku kwehydrolysis kunoita kuti ruvara ruonekwe, izvo zvinogona kushandiswa kuona kuvapo kwetupukanana utwu mumienzaniso yekiriniki.

Muenzaniso weChigadzirwa

Kurongedza Chigadzirwa:

Mamwe Mashoko:

Kuumbwa	C12H15NO8
Kuyedza	99%
Chitarisiko	Upfu hweyero yakapfava
Nhamba yeCAS	2816-24-2
Kurongedza	Diki uye rakakura
Hupenyu hweSherufu	makore maviri
Kuchengetera	Chengeta munzvimbo inotonhorera uye yakaoma
Chitupa	ISO.