3-[(3-Cholanidopropyl)dimethylammonio]-1-propanesulfonate CAS:75621-03-3

CHAPS (3--[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate) inowanzoshandiswa detergent mubiochemistry uye molecular biology.Iyo zwitterionic detergent, zvichireva kuti ine zvese zvakanaka uye zvisina kunaka boka.

CHAPS inozivikanwa nekugona kwayo kunyungudutsa uye kudzikamisa membrane mapuroteni, zvichiita kuti ishande kune akasiyana maapplication akadai sekubvisa mapuroteni, kucheneswa, uye hunhu.Iyo inovhiringidza lipid-protein kuwirirana, zvichibvumira membrane mapuroteni kuti abudiswe munzvimbo yavo yekuzvarwa.

Kusiyana nezvimwe zvekugezesa, CHAPS yakapfava uye haitore mapuroteni mazhinji, zvichiita kuti ive sarudzo yakanaka kwazvo yekuchengetedza chimiro cheprotein uye kushanda panguva yekuyedza.Inogonawo kubatsira kudzivirira kuunganidzwa kweprotein.

CHAPS inowanzoshandiswa muhunyanzvi seSDS-PEJI (sodium dodecyl sulfate polyacrylamide gel electrophoresis), isoelectric inotarisa, uye Western blotting.Iyo inowanzoshandiswa muzvidzidzo zvinosanganisira membrane-yakasungwa enzymes, chiratidzo chekufambisa, uye protein-lipid kupindirana.

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Application uye Effect

Protein Extraction: CHAPS inowanzoshandiswa kubvisa membrane mapuroteni kubva kubiological samples.Inobatsira solubilize mapuroteni aya uye kuchengetedza chimiro chavo chekuzvarwa.

Kucheneswa kweprotein: CHAPS inoshandiswa mumhando dzakasiyana dzemapuroteni ekuchenesa, senge affinity chromatography.Inogona kuwedzerwa kune ekucheneswa buffers kunyungudutsa uye kudzikamisa membrane mapuroteni panguva yekucheneswa maitiro.

Protein Characterization: CHAPS inowanzoshandiswa muzvidzidzo zvinosanganisira hunhu hwemembrane proteins.Inobatsira kuchengetedza chimiro cheprotein uye kushanda panguva yekuyedza maitiro senge enzyme chiitiko assays, protein-protein kupindirana, uye spectroscopic ongororo.

Membrane Protein Zvidzidzo: Membrane mapuroteni anoita mabasa akakosha mumaserura mazhinji maitiro.CHAPS inowanzoshandiswa mukutsvagisa ine chekuita nechiratidzo chekufambisa, ion chiteshi basa, protein-lipid kupindirana, uye membrane protein crystallization.

Electrophoresis: CHAPS inoshandiswa muhunyanzvi seSDS-PEJI uye isoelectric inotarisa kunyungudutsa membrane mapuroteni uye kufambisa kupatsanurwa kwavo uye kuongororwa.

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Ruzivo Rwokuwedzera:

Composition	C32H58N2O7S
Assay	99%
Chitarisiko	White crystalline powder
CAS Nha.	75621-03-3
Packing	Zvidiki uye zvakawanda
Sherufu Hupenyu	2 years
Storage	Chengetedza munzvimbo inotonhorera uye yakaoma
Certification	ISO.