3-[(3-Cholanidopropyl)dimethylammonio]-1-propanesulfonate CAS:75621-03-3

CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate) imushonga unoshandiswa zvakanyanya mu biochemistry uye molecular biology. Imushonga unogadzirwa nezwitterionic, zvichireva kuti une boka rine chaji yakanaka uye yakaipa.

CHAPS inozivikanwa nekukwanisa kwayo kunyungudutsa nekudzikamisa mapuroteni emukati, zvichiita kuti ishande mumabasa akasiyana-siyana akadai sekubviswa kweprotein, kucheneswa, uye kutsanangurwa kwemavara. Inokanganisa kudyidzana kwemafuta nemapuroteni, zvichiita kuti mapuroteni emukati abudiswe ari mumamiriro awo ekuzvarwa.

Kusiyana nedzimwe sipo, CHAPS haina kunyorova uye haikanganisi mapuroteni akawanda, zvichiita kuti ive sarudzo yakanaka yekuchengetedza chimiro cheprotein uye mashandiro ayo panguva yekuedza. Inogonawo kubatsira kudzivirira kuungana kwemapuroteni.

CHAPS inowanzoshandiswa mumaitiro akaita seSDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis), isoelectric focusing, uye Western blotting. Inoshandiswawo kakawanda muzvidzidzo zvinosanganisira ma enzymes akabatana ne membrane, signal transduction, uye protein-lipid interactions.

Odha kana Kumbira Mutengo

Ruzivo rweChigadzirwa

Matagi eChigadzirwa

Kushandiswa uye Mhedzisiro

Kubviswa kweMapuroteni: CHAPS inowanzoshandiswa kubvisa mapuroteni emukati kubva muzviyero zvehupenyu. Inobatsira kunyungudutsa mapuroteni aya uye kuchengetedza chimiro chawo chepakutanga.

Kuchenesa Mapuroteni: CHAPS inoshandiswa mumhando dzakasiyana dzekuchenesa mapuroteni, dzakadai se affinity chromatography. Inogona kuwedzerwa kuma purification buffers kuti inyungudike uye igare yakasimba panguva yekuchenesa.

Kutsanangura Mapuroteni: CHAPS inowanzoshandiswa muzvidzidzo zvinosanganisira kutsanangura mapuroteni emukati memuviri. Inobatsira kuchengetedza chimiro chepuroteni uye mashandiro panguva yekuedza kwakadai sekuyedza mashandiro eenzyme, kudyidzana kwemapuroteni nemapuroteni, uye kuongorora kwespectroscopic.

Zvidzidzo zveMapuroteni eNyemba: Mapuroteni eNyemba anoita basa rakakosha mumabasa mazhinji emasero. CHAPS inowanzoshandiswa mukutsvagisa kwakabatana nekuchinja kwechiratidzo, mashandiro eion channel, kudyidzana kwemapuroteni nemafuta, uye crystallization yeprotein yeNyemba.

Electrophoresis: CHAPS inoshandiswa muhunyanzvi hwakaita seSDS-PAGE uye isoelectric inotarisa mukunyungudutsa mapuroteni emembrane uye kuita kuti aparadzaniswe uye aongororwe.

Kurongedza Chigadzirwa:

Mamwe Mashoko:

Kuumbwa	C32H58N2O7S
Kuyedza	99%
Chitarisiko	Upfu hwekristalline chena
Nhamba yeCAS	75621-03-3
Kurongedza	Diki uye rakakura
Hupenyu hweSherufu	makore maviri
Kuchengetera	Chengeta munzvimbo inotonhorera uye yakaoma
Chitupa	ISO.