5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide CAS:4264-82-8

5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide (X-Gluc) inonzi chromogenic substrate inowanzoshandiswa pakutsvaga beta-glucuronidase (GUS) basa.GUS ienzyme inowanikwa muzvisikwa zvakasiyana-siyana, kusanganisira mabhakitiriya, zvirimwa, uye mhuka dzinoyamwisa.X-Gluc inowanzoshandiswa muGUS reporter assays uye molecular biology research.

Iko kushandiswa kukuru kweX-Gluc kuri mune histochemical staining matekiniki, kwaanogona kuona matauriro uye chiitiko cheGUS enzyme.Iyi substrate inogoneka sero uye inova hydrolyzed neGUS, zvichikonzera kuumbwa kweblue precipitate kana isinganyungudika chigadzirwa.Uku kusvibiswa kwebhuruu kunobvumira vaongorori kuti vaone uye vatore chiitiko cheGUS mumaseru, matishu, uye zvipenyu zvese.

X-Gluc inogona zvakare kushandiswa muhuwandu hwekuyedza kuyera GUS enzyme chiitiko.Kusimba kweruvara rwebhuruu kana huwandu hwechigadzirwa hwakaumbwa hunogona kuwiriraniswa nechiyero cheGUS kutaura kana kuita kwayo enzymatic.

Pamusoro pezvo, X-Gluc yakashandiswa mukutsvagisa genetic tsvakiridzo kuti idzidze mataurirwo ejeni, chiitiko chekusimudzira, uye shanduko yezvirimwa.Yakashandiswawo mumabhakitiriya masisitimu ekugadzira uye kuona mapuroteni eGUS fusion.