DDT CAS:3483-12-3 Mugadziri Mutengo Unoratidzwa Mufananidzo

DDT CAS:3483-12-3 Mutengo wemugadziri

DL-Dithiothreitol, inozivikanwawo seDTT, inhengo inodzikisa inowanzoshandiswa mukutsvakurudza kwebiochemical uye molecular biology.Imorekuru diki rine thiol (sarufa-ine) boka pamagumo ega ega.

DTT inowanzoshandiswa kuputsa zvisungo zve disulfide mumapuroteni, izvo zvinobatsira kuvhura kana kuti denature.Uku kudzikiswa kwezvisungo zvedisulfide kwakakosha mumatanho akasiyana emurabhoritari senge protein kucheneswa, gel electrophoresis, uye zvidzidzo zveprotein chimiro.DTT inogona zvakare kushandiswa kuchengetedza thiol mapoka uye kudzivirira oxidation panguva yekuedza maitiro.

DTT inowanzowedzerwa kumhinduro dzekuyedza muzvikamu zvidiki, uye kuita kwayo kunoenderana nekuvapo kweokisijeni.Zvakakosha kubata DTT zvakanyatsonaka sezvo inonzwa kumhepo, kupisa, uye unyoro, izvo zvinogona kuderedza kushanda kwayo.

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Application uye Effect

Kuderedzwa kweDisulfide Bonds: DTT inonyanya kushandiswa kuputsa zvisungo zve disulfide, izvo zviri covalent zvisungo zvinoumbwa pakati pemasara maviri ecysteine mumapuroteni.Nekudzikisa zvisungo izvi, DTT inobatsira denature mapuroteni, ichigonesa kudzidza kwechimiro chavo uye basa.

Protein Folding: DTT inogona kubatsira mukupeta mapuroteni akakodzera nekudzivirira kuumbwa kwakashata disulfide bond.Iyo inoderedza chero isiri-yekuzvarwa disulfide zvisungo zvinogona kuumba panguva yeprotein kupeta, zvichibvumira puroteni kuti itore hunhu hwayo.

Enzyme Chiitiko: DTT inogona kumisa mamwe ma enzymes nekudzikisa chero inhibitory disulfide bond iripo.Uyezve, DTT inogona kudzivirira oxidation yezvakakomba cysteine residues, izvo zvingave zvakakosha kune enzyme basa.

Antibody Production: DTT inowanzowedzerwa kuderedza disulfide bond panguva yekugadzirwa kwemasoja ekudzivirira chirwere.Inobatsira kudzivirira kuumbwa kwezvisungo zvisina kururama disulfide, izvo zvinogona kutadzisa zvakakodzera antigen kusunga.

Kusimbisa Mapuroteni: DTT inogona kushandiswa kudzikamisa mapuroteni nekudzivirira oxidation yavo kana kuunganidzwa.Inobatsira kuchengetedza yakaderedzwa mamiriro emapuroteni panguva yekuchengetedza uye maitiro ekuedza.

Kuderedza maAgents muMolecular Biology: DTT inowanzoshandiswa mumhando dzakasiyana-siyana dzebhaibheri biology dzakadai seDNA sequencing, PCR, uye kucheneswa kweprotein.Inogona kubatsira kuchengetedza mamiriro akaderedzwa ezvikamu zvakakosha, kuve nechokwadi chemhedzisiro yekuedza.

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Ruzivo Rwokuwedzera:

Composition	C4H10O2S2
Assay	99%
Chitarisiko	Upfu chena
CAS Nha.	3483-12-3
Packing	Zvidiki uye zvakawanda
Sherufu Hupenyu	2 years
Storage	Chengetedza munzvimbo inotonhorera uye yakaoma
Certification	ISO.