DDT CAS:3483-12-3 Mutengo wemugadziri

DL-Dithiothreitol, inozivikanwawo seDTT, chinhu chinoderedza huwandu hwemasero mumuviri chinowanzoshandiswa muongororo ye biochemical ne molecular biology. Imorekuru diki ine boka re thiol (rine sarufa) kumagumo ese.

DTT inowanzo shandiswa kupwanya ma disulfide bonds mumapuroteni, izvo zvinobatsira kuavhura kana kuabvisa. Kudzikiswa uku kwe disulfide bonds kwakakosha mumabasa akasiyana-siyana erabhoritari akadai sekuchenesa mapuroteni, gel electrophoresis, uye zvidzidzo zvemagadzirirwo emapuroteni. DTT inogona zvakare kushandiswa kudzivirira mapoka e thiol uye kudzivirira oxidation panguva yemaitiro ekuyedza.

DTT inowanzo wedzerwa mumhinduro dzekuedza kana iri diki, uye mashandiro ayo anoenderana nekuvapo kweokisijeni. Zvakakosha kubata DTT nokungwarira sezvo inonzwa mhepo, kupisa, uye hunyoro, izvo zvinogona kuderedza kushanda kwayo.

Odha kana Kumbira Mutengo

Ruzivo rweChigadzirwa

Matagi eChigadzirwa

Kushandiswa uye Mhedzisiro

Kuderedza Mabond eDisulfide: DTT inonyanya kushandiswa kupwanya mabond edisulfide, ayo ari mabond ecovalent anoumbwa pakati pemaresidues maviri ecysteine mumapuroteni. Nekuderedza mabond aya, DTT inobatsira kuderedza mapuroteni, zvichiita kuti pave nekudzidza nezvemaumbirwo awo uye mashandiro awo.

Kupeta Mapuroteni: DTT inogona kubatsira mukupeta mapuroteni zvakanaka nekudzivirira kuumbwa kwedisulfide bond isina kunaka. Inoderedza chero zvisungo zvedisulfide zvisiri zvemuno zvinogona kuumbwa panguva yekupeta mapuroteni, zvichiita kuti puroteni itore chimiro chayo chemuno.

Basa reEnzayimu: DTT inogona kumutsa mamwe maenzyme nekuderedza chero zvisungo zve disulfide zvinodzivirira zviripo. Pamusoro pezvo, DTT inogona kudzivirira oxidation ye critical cysteine residues, izvo zvingave zvichidikanwa pabasa reenzayimu.

Kugadzirwa kweAntibody: DTT inowanzo wedzerwa kuderedza disulfide bonds panguva yekugadzirwa kweantibodies. Inobatsira kudzivirira kuumbwa kwe disulfide bonds dzisina kururama, izvo zvinogona kukanganisa kubatana kweantigen kwakakodzera.

Kusimbisa Mapuroteni: DTT inogona kushandiswa kusimbisa mapuroteni nekudzivirira kuoxidation kana kuungana kwawo. Inobatsira kuchengetedza mamiriro ekudzikira kwemapuroteni panguva yekuchengetwa uye yekuedza.

Zvinhu Zvinoderedza Kushandiswa muMolecular Biology: DTT inowanzoshandiswa mumhando dzakasiyana dzemamorekuru ebiology dzakadai seDNA sequencing, PCR, uye protein purification. Inogona kubatsira kuchengetedza mamiriro ezvikamu zvakakosha, zvichiita kuti pave nemigumisiro yakanaka kwazvo mukuyedza.

Muenzaniso weChigadzirwa

Kurongedza Chigadzirwa:

Mamwe Mashoko:

Kuumbwa	C4H10O2S2
Kuyedza	99%
Chitarisiko	Upfu chena
Nhamba yeCAS	3483-12-3
Kurongedza	Diki uye rakakura
Hupenyu hweSherufu	makore maviri
Kuchengetera	Chengeta munzvimbo inotonhorera uye yakaoma
Chitupa	ISO.