5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide ikamu rinoshandiswa muzvidzidzo zvakasiyana-siyana zvebiochemical, kunyanya pakuona uye kuona kwekuita kwe enzyme.Iyo substrate inogona kuve hydrolyzed nema enzymes chaiwo, zvichikonzera kuburitswa kwechigadzirwa chine ruvara kana fluorescent.
Mushonga uyu unowanzoshandiswa mukuedza kuona kuvepo uye kuita kwema enzymes akadai beta-galactosidase uye beta-glucuronidase.Aya ma enzymes anotsemura mapoka eacetyl uye glucosaminide kubva kune substrate, zvichiita kuti paumbwe chromophore yebhuruu kana yegirini.
Chimiro chakasiyana che5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide inobvumira kuonekwa nyore uye quantification yebasa re enzyme.Kushandiswa kwayo mumhando dzakasiyana dzekuyedza, kusanganisira histochemistry, immunohistochemistry, uye cell-based assays, zvakabatsira mukunzwisisa kuri nani kwemabasa ee enzyme.