5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide CAS:4264-82-8

5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide chinhu chinoshandiswa muzvidzidzo zvakasiyana-siyana zve biochemical, kunyanya pakuona uye kuona mashandiro ema enzyme. I substrate inogona kunyungudutswa nema enzyme chaiwo, zvichikonzera kuburitswa kwechinhu chine ruvara kana fluorescent.

Mushonga uyu unoshandiswa zvakanyanya mukuyedza kuona kuvapo uye kushanda kwema enzyme akadai se beta-galactosidase ne beta-glucuronidase. Ma enzyme aya anobvisa mapoka e acetyl ne glucosaminide kubva mu substrate, zvichikonzera kuumbwa kwe chromophore yebhuruu kana yegirini.

Maumbirwo akasiyana e5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide anoita kuti zvive nyore kuona uye kuyera mashandiro ema enzyme. Kushandiswa kwayo mumaitiro akasiyana-siyana ekuyedza, anosanganisira histochemistry, immunohistochemistry, uye masero, kwakabatsira mukunzwisisa zviri nani mashandiro ema enzyme.

Odha kana Kumbira Mutengo

Ruzivo rweChigadzirwa

Matagi eChigadzirwa

Kushandiswa uye Mhedzisiro

5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide (X-Gluc) chinhu chinowanzo shandiswa pakuongorora mashandiro e beta-glucuronidase (GUS). GUS ienzyme inowanikwa muzvinhu zvipenyu zvakasiyana-siyana, kusanganisira mabhakitiriya, zvirimwa, uye mhuka dzinoyamwisa. X-Gluc inowanzoshandiswa mukuongorora kweGUS reporter assays uye kutsvakurudza kwe molecular biology.

Kushandiswa kukuru kweX-Gluc kuri muhunyanzvi hwekunyora mavara ehistochemical, uko kunogona kuona kuratidzwa uye kushanda kweenzyme yeGUS. Iyi substrate inopindika mumasero uye inonyungudika neGUS, zvichikonzera kuumbwa kweblue precipitate kana chigadzirwa chisinganyungudike. Kunyora mavara aya ebhuruu kunobvumira vaongorori kuona uye kuona mashandiro eGUS mumasero, munyama, uye zvipenyu zvese.

X-Gluc inogona kushandiswawo mukuyedza huwandu hwema enzymes eGUS. Kusimba kweruvara rwebhuruu kana huwandu hwechigadzirwa chinogadzirwa zvinogona kuenderana nehuwandu hweGUS expression kana mashandiro ayo ema enzymes.

Pamusoro pezvo, X-Gluc yakashandiswa mukutsvagisa majini emiti kudzidza nezvekugadzirwa kwemajini, mashandiro ekusimudzira, uye kushandurwa kwemiti. Yakashandiswawo mumasisitimu emabhakitiriya ekugadzira nekuburitsa mapuroteni eGUS fusion.

Kurongedza Chigadzirwa:

Mamwe Mashoko:

Kuumbwa	C16H18BrClN2O6
Kuyedza	99%
Chitarisiko	Upfu chena
Nhamba yeCAS	4264-82-8
Kurongedza	Diki uye rakakura
Hupenyu hweSherufu	makore maviri
Kuchengetera	Chengeta munzvimbo inotonhorera uye yakaoma
Chitupa	ISO.