Tris(hydroxymethyl)nitromethane CAS:126-11-4

Mhedzisiro:

Buffering Capacity: Tris inoshanda senge inobudirira buffering agent nekuda kwekugona kwayo kugamuchira kana kupa maproton, kuchengetedza yakagadzikana pH renji mumhinduro.Iyo inowanzo shandiswa sechinhu chekutanga mu buffer masisitimu kudzikamisa iyo pH yemasampuri ezvinyorwa uye maitiro.

Applications:

Molecular Biology: Tris inoshandiswa zvakanyanya seyakavharisa mumiriri mune dzakasiyana siyana mamorekuru biology matekiniki, anosanganisira DNA uye RNA yekuzviparadzanisa nevamwe, PCR, gel electrophoresis, uye protein kucheneswa.Inobatsira kuchengetedza yakagadzikana pH nharaunda, ichibvumira mamiriro akakwana ekuita enzymatic uye kusangana kwema molecular.

Sero Tsika: Tris inowanzo shandiswa musero tsika midhiya kuchengetedza inogara pH uye osmotic chiyero, ichitsigira hutano hwesero kukura uye kushanda.

Protein Chemistry: Tris inoshandiswa mukuedza kweprotein chemistry, senge protein solubilization, protein stability assays, uye protein-ligand inosunga zvidzidzo.Inobatsira kuchengetedza yaidiwa pH renji, kuve nechokwadi chakakodzera protein kupeta uye chiitiko.

Enzymology: Tris inoshandiswa mune dzakasiyana siyana enzymatic assays kukwirisa iyo pH mamiriro anodiwa kune enzymatic chiitiko.Iyo inovimbisa yakavimbika uye inobereka mhedzisiro, ichigonesa kuyerwa kwakaringana kwe enzyme kinetics uye inhibition zvidzidzo.

Biochemical Assays: Tris inoshandiswa sechikamu mune dzakawanda biochemical assays nekuda kweiyo buffering zvimiro.Iyo inochengetedza inogara pH panguva ye colorimetric, spectrophotometric, uye enzymatic assays, ichiwedzera chokwadi uye kuvimbika kwemhedzisiro.