3-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE CAS:3150-25-2

Kuonekwa kwebeta-galactosidase chiitiko: ONPG inowanzo shandiswa kuona kuvepo uye chiitiko che beta-galactosidase mune akasiyana biological samples, senge mabhakitiriya tsika kana sero lysates.Kugadzirwa kwe o-nitrophenol, ine ruvara rweyero, inogona kuyerwa nyore nyore uchishandisa spectrophotometer.

Zvidzidzo zveGene kutaura: ONPG inowanzoshandiswa mukutsvagisa mamorekuru biology kudzidza mataurirwo ejene.Nekusanganisa mutsigiri wejini rekufarira neiyo gene encoding beta-galactosidase, vaongorori vanogona kuyera chiitiko chemutsigiri uyu nekuwedzera ONPG uye nekuenzanisa mhedzisiro ye-o-nitrophenol kugadzirwa.Iyi nzira, inozivikanwa se beta-galactosidase reporter assay, inopa ruzivo pamusoro pekuita kwekunyora kwejini.

Kuzivikanwa kwebhakitiriya: Mamwe mabhakitiriya anoburitsa beta-galactosidase, asi mamwe haaite.ONPG inogona kushandiswa pamwe chete nedzimwe bvunzo dzebiochemical kuona mhando dzebhakitiriya zvichienderana nekugona kwavo kuita hydrolyze ONPG.Iyi nzira inowanzoshandiswa mumakiriniki ekuongorora uye microbiology marabhoritari.

Kuongororwa kwe enzyme inhibitors kana activators: ONPG inogona kushandiswa kuongorora makomisheni anogadzirisa chiitiko che beta-galactosidase.Nekuyera chiitiko che enzyme pamberi peakasiyana makomisheni, vaongorori vanogona kuona zvinogona inhibitors kana activator izvo zvinogona kuongororwa zvakare nezve kugona kwavo kwekurapa.