3-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE CAS:3150-25-2

3-Nitrophenyl-beta-D-galactopyranoside (ONPG) imhando yechinhu chinowanzoshandiswa mukuyedza ma enzymes kuti chione uye chiyere mashandiro e beta-galactosidase. Kana beta-galactosidase iripo uye ichishanda, inonyungudutsa ONPG, ichiburitsa chinhu chine ruvara rweyero chinonzi 3-nitrophenol. Kusimba kweruvara rweyero rinogadzirwa kunogona kuyerwa ne spectrophotometric, zvichibvumira kuyerwa kwemaitiro e beta-galactosidase. ONPG inowanzoshandiswa muongororo ye molecular biology ne microbiology, pamwe nekuongorora kwekiriniki, kudzidza nezvemagene, mashandiro eprotein, kuzivikanwa kwebhakitiriya, uye kugara zvakanaka kwemasero.

Odha kana Kumbira Mutengo

Ruzivo rweChigadzirwa

Matagi eChigadzirwa

Kushandiswa uye Mhedzisiro

Kuonekwa kwemaitiro e beta-galactosidase: ONPG inowanzoshandiswa kuona kuvapo uye mashandiro e beta-galactosidase mumhando dzakasiyana dzezvipenyu, dzakadai semabhakitiriya kana masero e lysates. Kugadzirwa kwe o-nitrophenol, ine ruvara rweyero, kunogona kuyerwa nyore nyore uchishandisa spectrophotometer.

Zvidzidzo zvemajini: ONPG inowanzoshandiswa mukutsvagisa kwemakemikari emuviri kudzidza magene. Nekubatanidza chinhu chinokurudzira gene rinofarira negene rinonyora beta-galactosidase, vaongorori vanogona kuyera mashandiro emusimudziri uyu nekuwedzera ONPG uye kuyerwa kwekugadzirwa kweo-nitrophenol kunobva kwaitika. Nzira iyi, inozivikanwa se beta-galactosidase reporter assay, inopa ruzivo nezve transcriptional activity yegene.

Kuzivikanwa kwebhakitiriya: Mamwe mabhakitiriya anogadzira beta-galactosidase, asi mamwe haaite. ONPG inogona kushandiswa pamwe chete nedzimwe bvunzo dzemakemikari kuona mhando dzebhakitiriya zvichienderana nekukwanisa kwavo hydrolyze ONPG. Nzira iyi inowanzoshandiswa mukuongorora kwekiriniki uye mumarabhoritari e microbiology.

Kuongororwa kwemakemikari anodzivirira kana activator: ONPG inogona kushandiswa kuongorora makemikari anochinja mashandiro e beta-galactosidase. Nekuyera mashandiro e enzyme pamberi pemakemikari akasiyana, vaongorori vanogona kuona makemikari anogona kudzivirira kana activator anogona kuongororwa zvakanyanya kuti aone kugona kwawo kurapa.

Kurongedza Chigadzirwa:

Mamwe Mashoko:

Kuumbwa	C12H15NO8
Kuyedza	99%
Chitarisiko	Chenaupfu
Nhamba yeCAS	3150-25-2
Kurongedza	Diki uye rakakura
Hupenyu hweSherufu	makore maviri
Kuchengetera	Chengeta munzvimbo inotonhorera uye yakaoma
Chitupa	ISO.