3,3,5,5 -Tetramethylbenzidine dihydrochloride hydrate CAS:207738-08-7

3,3', 5,5'-Tetramethylbenzidine dihydrochloride (TMB) imhando inoshandiswa zvakanyanya mu enzyme-linked immunosorbent assays (ELISAs) nedzimwe biochemical assays. Kana TMB ikasanganiswa ne enzyme horseradish peroxidase (HRP) paine hydrogen peroxide, inoburitsa ruvara rwebhuruu. Kuchinja kweruvara uku kunoshandiswa kuona kuvapo kwema antibodies ane label yeHRP kana mamwe mapuroteni, kazhinji zvichiratidza kuvapo kweantigen chaiyo mumuenzaniso. Ruvara rwebhuruu rwunogadzirwa nemhinduro runowanzo verengerwa uchishandisa spectrophotometer, iyo inobvumira kuyerwa kwesimba rekuchinja kweruvara uye inopa nzira yekubatanidza simba reruvara nehuwandu hwemorekuru iri mumuenzaniso. TMB ine mashandisirwo akawanda mukuongorora, kutsvagisa, uye kugadzira mishonga, kunyanya mukuona ma antibodies, mahormone, ma cytokines, uye mamwe ma biomolecule. Kunzwa kwayo, kugadzikana, uye nyore kushandisa kunoita kuti ive chishandiso chakakosha munzvimbo dzakasiyana dzerabhoritari dzekuona nekuyera mamorekuru chaiwo.