4-Nitrophenyl-2-acetamido-2-deoxy-β-D-glucopyranoside CAS:3459-18-5

4-Nitrophenyl-2-acetamido-2-deoxy-β-D-glucopyranoside ikemikari inoshandiswa zvakanyanya muongororo ye biochemical uye molecular biology. I substrate inozivikanwa se pNAG, inoshandiswa zvakanyanya mukuyedza enzymes kuona nekuyera mashandiro ema enzymes anonyungudutsa β-D-glucoside bonds.

pNAG haina ruvara uye haina fluorescent, asi kana yashandiswa nema enzymes chaiwo, inogadzira chinhu chine ruvara rweyero, p-nitrophenol. Hunhu uhwu hunoita kuti pNAG ibatsire pakuona uye kuyedza mashandiro ema enzymes akadai se β-glucosidases, ayo anokonzera kutsemuka kwe β-D-glucoside bonds.

Odha kana Kumbira Mutengo

Ruzivo rweChigadzirwa

Matagi eChigadzirwa

Kushandiswa uye Mhedzisiro

Substrate yeEnzyme: pNAG inoshandiswa zvakanyanya sechikamu che enzymes dzakasiyana-siyana dzinobatanidzwa mukubviswa kwe β-D-glucoside bonds. Kana ma enzymes aya akatsemura pNAG molecule, inoburitsa p-nitrophenol. Izvi zvinobvumira vaongorori kuyera uye kuyera mashandiro e enzyme.

Kuyedzwa kweEnzyme Activity: Kunyungudika kwepNAG nema enzymes chaiwo kunogona kuonekwa uye kuyerwa ne spectrophotometric. Izvi zvinoita kuti pNAG ikodzere kuyedzwa kwe enzyme activity, uko huwandu hwe p-nitrophenol hunogadzirwa hunoenderana zvakananga nebasa re enzyme.

Kuongorora Kwemhando Yepamusoro: pNAG inowanzoshandiswa mukuongorora kwemhando yepamusoro kuti ione uye ione zvinodzivirira enzyme kana zvinokonzeresa. Nekuongorora mhedzisiro yemakemikari akasiyana pabasa re enzyme, vaongorori vanogona kuona zvinogona kushandiswa pakurapa kana kugadzirisa mashandiro e enzyme.

Zvidzidzo zveKuratidza Majini: pNAG inoshandiswawo mukutsvagisa kwebiology yemamorekuru kudzidza kuratidzwa kwemajini uye kurongeka kwawo. Nekuyera mashandiro emaenzymes chaiwo uchishandisa pNAG sechikamu, vaongorori vanogona kuongorora mhedzisiro yekuratidzwa kwemajini pabasa uye chiitiko chemaenzyme.

Kurongedza Chigadzirwa:

Mamwe Mashoko:

Kuumbwa	C14H18N2O8
Kuyedza	99%
Chitarisiko	Upfu chena
Nhamba yeCAS	3459-18-5
Kurongedza	Diki uye rakakura
Hupenyu hweSherufu	makore maviri
Kuchengetera	Chengeta munzvimbo inotonhorera uye yakaoma
Chitupa	ISO.