4-Nitrophenyl-alpha-D-galactopyranoside CAS:7493-95-0

Substrate ye enzymatic assays: 4-Nitrophenyl-alpha-D-glucopyranoside inowanzoshandiswa se substrate mu enzymatic assays kuyera basa re glycosidases uye mamwe ma enzymes anobatanidzwa mu carbohydrate metabolism.Aya ma enzymes anogona kutsemura chisungo pakati peglucose ne4-nitrophenyl boka, achiburitsa yero chigadzirwa chinonzi 4-nitrophenol.Nhamba ye4-nitrophenol inogadzirwa yakanyatsoenderana nekuita kwe enzyme, zvichiita kuti kuyerwa kwehuwandu hwekuita kwe enzyme.

Kuona basa re enzyme: Nekushandisa 4-Nitrophenyl-alpha-D-glucopyranoside se substrate, basa rema enzymes chaiwo rinogona kuyerwa.Semuenzaniso, glycosidase enzymes yakadai se beta-glucosidase, alpha-glucosidase, kana beta-galactosidase inogona kutsemura musanganiswa, ichibudisa 4-nitrophenol, iyo inogona kuonekwa photometrically.Iyi nzira inoshandiswa zvakanyanya mumhando dzakasiyana dzekurapa, mishonga, uye biotechnological application.

High-throughput screening: Nekuda kwekuenderana kwayo neotomatiki yekuona masisitimu, 4-Nitrophenyl-alpha-D-glucopyranoside inowanzoshandiswa mumhando yepamusoro yekuongorora.Iyi koniyuta inobvumira kukurumidza uye nekuita hunhu hwekuita enzyme, ichiita kuti ibatsire mukuwanikwa kwezvinodhaka, enzyme engineering, uye enzyme inhibition zvidzidzo.

Diagnostic applications: 4-Nitrophenyl-alpha-D-glucopyranoside inogona kushandiswa mumashandisirwo ekuongorora kuona kuvepo kana basa rema enzymes ane chekuita nezvirwere zvakasiyana.Semuenzaniso, basa remamwe ma enzymes rinogona kushandiswa sechiratidzo chekuongorora kwezvirwere zvakasiyana, uye 4-Nitrophenyl-alpha-D-glucopyranoside inogona kushandiswa se substrate kuyera basa rema enzymes mumakiriniki samples.