FLUORESCEIN MONO-BETA-D- GALACTOPYRANOSIDE CAS:102286-67-9

Fluorescein mono-beta-D-galactopyranoside, inozivikanwawo seFMG, imusanganiswa we fluorescent unoshandiswa zvakanyanya se substrate mukuyedza kwakasiyana-siyana kwe biochemical uye cell biology. Inotorwa kubva ku methyl-beta-D-galactopyranoside nekuibatanidza ne fluorescein molecule.FMG inoshandiswa zvakanyanya kudzidza mashandiro e beta-galactosidase, enzyme inokonzeresa hydrolysis ye lactose kuita galactose ne glucose. Nekushandisa FMG se substrate, vaongorori vanogona kutarisa mashandiro e enzyme e beta-galactosidase kuburikidza nekuyera fluorescence emission. Hydrolysis ye FMG ne beta-galactosidase inotungamira mukuburitswa kwe fluorescein, zvichikonzera kuwedzera kwechiratidzo che fluorescent chinogona kuverengwa. Iyi compound inoshandiswawo kuongorora kuzivikanwa kwema carbohydrate uye kudyidzana. FMG inogona kushandiswa se molecular probe kudzidza kubatana kwema lectins (mapuroteni anobatana nema carbohydrates) kuma galactose-containing carbohydrates. Kusungana kweFMG-lectin complexes kunogona kuonekwa uye kuyerwa zvichibva pakuchinja kwekubuda kwefluorescence. Kazhinji, FMG chishandiso chinoshanda zvakasiyana-siyana mukudzidza mashandiro ema enzyme uye kuziva makabhohaidhiretsi, zvichipa nzira iri nyore uye inonzwisisika yekuyera fluorescence uye kuongorora maitiro aya ehupenyu.

Odha kana Kumbira Mutengo

Ruzivo rweChigadzirwa

Matagi eChigadzirwa

Kushandiswa uye Mhedzisiro

Fluorescein mono-beta-D-galactopyranoside (FMG) imorekuru inowanzoshandiswa mukutsvagisa kwezvebhayoloji sechikamu chekuongorora kuvapo uye kushanda kwe beta-galactosidase enzyme. FMG inobva mushuga lactose uye inobatanidzwa nemorekuru ye fluorescein.

Mhedzisiro huru yeFMG ndeyekuti inonyungudika zvakanyanya ne beta-galactosidase, enzyme inoputsa lactose kuita galactose neglucose. Kunyungudika uku kweFMG kunoita kuti fluorescein iburitswe, iyo inoburitsa chiratidzo chakasimba che fluorescence.

Kushandiswa kukuru kweFMG ndekwekuona nekuyera mashandiro e beta-galactosidase mumasampuli akasiyana-siyana. Iyi enzyme inowanikwa muzvinhu zvipenyu zvakawanda, kusanganisira mabhakitiriya nemasero emhuka dzinoyamwisa, uye mashandiro ayo anogona kuratidza maitiro akasiyana-siyana emasero uye nzira dzekuchinja mashandiro emuviri.

Nekushandisa FMG sechikamu chepasi, basa re beta-galactosidase rinogona kuyerwa nekutarisa fluorescence inoburitswa ne fluorescein yakasunungurwa. Kuyera uku kunogona kuitwa mumhando dzakasiyana dzekuyedza, kusanganisira bvunzo dze in vitro uye zvidzidzo zve live cell imaging.

Uyezve, FMG inogona kushandiswa sechishandiso chekudzidza kupararira uye nzvimbo ye beta-galactosidase mukati memasero. Nekushandisa nzira dze fluorescent microscopy, vaongorori vanogona kuona fluorescence inoburitswa ne FMG panguva ye hydrolysis, zvichivabvumira kutevedzera mashandiro e beta-galactosidase munzvimbo nenzvimbo.

Muenzaniso weChigadzirwa

Kurongedza Chigadzirwa:

Mamwe Mashoko:

Kuumbwa	C26H22O10
Kuyedza	99%
Chitarisiko	Upfu chena
Nhamba yeCAS	102286-67-9
Kurongedza	Diki uye rakakura
Hupenyu hweSherufu	makore maviri
Kuchengetera	Chengeta munzvimbo inotonhorera uye yakaoma
Chitupa	ISO.