4-NITROPHENYL-ALPHA-D-MANNOPYRANOSIDE CAS:10357-27-4

Enzyme substrates: 4NPM inogona kushandiswa se substrate yema enzymes akasiyana-siyana, kusanganisira glycosidases uye ma enzymes ane hukama.Aya ma enzymes anotsemura chisungo cheglycosidic pakati pemannose ne4NPM, zvichikonzera kuburitswa kweiyo nitrophenyl moiety.Hukuru hwe substrate hydrolysis hunogona kuyerwa spectrophotometrically nekutarisa kunyura kweboka renitrophenyl rakaburitswa pane imwe wavelength.Izvi zvinobvumira vatsvakurudzi kuti vaongorore basa re enzyme uye kinetics.

Kuongororwa kwecarbohydrate metabolism: Nekushandisa 4NPM se substrate, vaongorori vanogona kudzidza basa rema enzymes anobatanidzwa mucarbohydrate metabolism, senge alpha-mannosidases.Aya ma enzymes hydrolyze glycosidic bonds mumannose-ine makomisheni, uye basa ravo rinogona kuyerwa nekutarisa kuburitswa kweiyo nitrophenyl moiety kubva ku4NPM.

Glycosylation zvidzidzo: 4NPM inogona zvakare kushandiswa mukuyedza kuongorora ma enzymes anobatanidzwa mune glycosylation maitiro.Glycosylation inzira yekubatanidza shuga mamorekuru kumapuroteni kana mamwe mamorekuru, uye ma enzymes mazhinji anobatanidzwa mukuita uku.Nekushandisa 4NPM seyakagashira substrate, vaongorori vanogona kuyera kuendeswa kweshuga moiety ku4NPM nema enzymes chaiwo anobatanidzwa mukuita kweglycosylation.

Kuongororwa kwe enzyme inhibitors kana activators: 4NPM inogona kushandiswa mu-high-throughput screening assays kuti ione makemikari anodzivisa kana kushandura ma enzymes chaiwo.Nekuongorora mhedzisiro yemakomponi ebvunzo pane hydrolysis kana kugadziridzwa kwe4NPM neinonangwa enzymes, vaongorori vanogona kuona vangangove mishonga yekurapa kana inobatsira kemikari probe yekudzidza enzyme basa.