Phenylgalactoside CAS:2818-58-8

Mhedzisiro pakushanda kwema enzyme: Phenylgalactoside inowanzo shandiswa kuyera mashandiro e enzyme β-galactosidase. Kana phenylgalactoside ikasanganiswa ne β-galactosidase, inoburitsa p-nitrophenol. Kuunganidzwa kwe p-nitrophenol kunogona kuyerwa nehuwandu, zvichipa ruzivo rwekushanda kwe β-galactosidase. Mhedzisiro iyi inoshandiswa mukushandiswa kwakadai sekuyedza ma enzyme uye masisitimu ekuongorora.

Kuongororwa kwemaitiro emajini: Phenylgalactoside inowanzoshandiswa mukuyedza kwemakemikari ebhayoloji kuti idzidze nezvemaitiro emajini. Jini relacZ, iro rinobatanidza β-galactosidase, rinowanzo sanganiswa nemaitiro ekutonga emamwe majini anodiwa. Kuonekwa kwejini relacZ uye hydrolysis yephenylgalactoside neβ-galactosidase zvinogona kuratidza maitiro ekutaura uye mwero wejini riri kudzidzwa.

Masisitimu ekuongorora: Phenylgalactoside chinhu chakakosha mumasisitimu ekuongorora anoshandisa β-galactosidase activity. Muenzaniso unozivikanwa zvikuru inzira yekuongorora yebhuruu-chena, iyo inoshandiswa kuona masero akagadziriswa kana akachinjwa mukuyedza kwebiology yemamorekuru. Makoroni akabudirira kutora DNA yakagadziriswa kana akagadziriswazve nemajini acharatidza β-galactosidase, zvichikonzera hydrolysis yephenylgalactoside uye kuumbwa kweruvara rwebhuruu.

Kucheneswa kweprotein: Mune zvimwe zviitiko, phenylgalactoside inogona kushandiswa se ligand ye affinity chromatography kuchenesa mapuroteni anonyatsobatana kana kuti anomutswa ne β-galactosidase. Protein inodiwa inogona kunge iine affinity tag kana fusion tag ine β-galactosidase-binding domain. Nekupfuudza musanganiswa weprotein nepakoramu ine phenylgalactoside isina kufamba, protein inodiwa inogona kuchengetedzwa zvakasarudzika uye mushure mezvo isingashande.