4-Nitrophenyl beta-D-galactopyranoside CAS:200422-18-0

Mhedzisiro: ONPG i substrate yakanyatso shandiswa kuona kuvepo uye kuita kweiyo enzyme β-galactosidase.Kana β-galactosidase enzyme iripo uye ichishanda, inotsemura ONPG kuita zvigadzirwa zviviri: o-nitrophenol uye galactose derivative.Kusunungurwa kwe o-nitrophenol kunoguma nekushanduka kweruvara rweyero, iyo inogona kuyerwa uchishandisa spectrophotometer.

Chikumbiro: ONPG ine akati wandei maapplication mumolecular biology uye biochemistry research:

Kutsunga kwe β-galactosidase chiitiko: ONPG inowanzoshandiswa kuyera uye kuyera basa re β-galactosidase enzyme.Chiyero che o-nitrophenol kuumbwa, iyo yakanyatsoenderana neiyo enzyme chiitiko, inogona kuyerwa spectrophotometrically.

Gene kutaura uye mutemo: ONPG inowanzo shandiswa mukuyedza ine chekuita nejini kutaura uye zvidzidzo zvemitemo.Inowanzoshandiswa mufusion protein assays, senge inowanzoshandiswa lacZ fusion system, kudzidza kutaura kwemajini pasi pekutonga kwevanosimudzira.Iyo beta-galactosidase chiitiko chakayerwa uchishandisa ONPG chinopa manzwisisiro padanho rekutaura kwemajini.

Kuongorora kwe β-galactosidase chiitiko: ONPG inogona kushandiswa se colorimetric yekuongorora nzira mune recombinant DNA tekinoroji kuona kuvepo uye kushanda kweiyo LacZ gene, iyo encode β-galactosidase.Iyi nzira yekuongorora inobatsira mukuona ma clones ane gene rekufarira.

Enzyme kinetics zvidzidzo: ONPG inobatsirawo mukudzidza kinetics ye β-galactosidase enzyme.Nekuyera chiyero che enzyme-substrate reaction pane akasiyana substrate concentrations, zvinokwanisika kuona kinetic parameters seMichaelis-Menten constants (Km) uye yakanyanya reaction rates (Vmax).