4-Nitrophenyl beta-D-galactopyranoside CAS:200422-18-0

Mhedzisiro: ONPG chinhu chinoshandiswa zvakanyanya kuona kuvapo uye kushanda kweenzyme inonzi β-galactosidase. Kana enzyme yeβ-galactosidase iripo uye ichishanda, inocheka ONPG kuita zvigadzirwa zviviri: o-nitrophenol uye galactose derivative. Kusunungurwa kweo-nitrophenol kunoguma nekuchinja kweruvara rweyero, izvo zvinogona kuyerwa uchishandisa spectrophotometer.

Kushandisa: ONPG ine mashandisirwo akati wandei muongororo ye molecular biology uye biochemistry:

Kuona mashandiro eβ-galactosidase: ONPG inowanzoshandiswa kuyera nekuverenga mashandiro eenzyme yeβ-galactosidase. Mwero wekuumbwa kweo-nitrophenol, unoenderana zvakananga nebasa reenzyme, unogona kuyerwa ne spectrophotometric.

Kuratidzwa kwemajini uye mitemo: ONPG inowanzoshandiswa mukuyedza kwakabatana nekudzidza kwekugadzirwa kwemajini uye mitemo. Inowanzoshandiswa mukuyedza mapuroteni ekubatanidza, senge lacZ fusion system inowanzoshandiswa, kudzidza kuratidzwa kwemajini pasi pekutonga kwevanokurudzira. Basa re beta-galactosidase rakayerwa uchishandisa ONPG rinopa ruzivo rwehuwandu hwekugadzirwa kwemajini.

Kuongororwa kwe β-galactosidase activity: ONPG inogona kushandiswa senzira yekuongorora ine ruvara rwemavara mu recombinant DNA tekinoroji kuti ione kuvapo uye mashandiro eLacZ gene, iyo inochengetedza β-galactosidase. Iyi nzira yekuongorora inobatsira mukuona ma clones ane gene rekuda.

Zvidzidzo zveEnzyme kinetics: ONPG inobatsirawo pakudzidza kinetics ye β-galactosidase enzyme. Nekuyera mwero we enzyme-substrate reaction pahuwandu hwakasiyana hwe substrate, zvinokwanisika kuona kinetic parameters dzakadai seMichaelis-Menten constants (Km) uye maximum reaction rates (Vmax).