P-NITROPHENYL BETA-D-LACTOPYRANOSIDE CAS:4419-94-7

P-Nitrophenyl beta-D-lactopyranoside, inozivikanwawo sePNPG, chinhu chinowanzoshandiswa mukuyedza enzyme kuyera mashandiro e beta-galactosidase, enzyme inobatanidzwa mu metabolism ye carbohydrate. PNPG chinhu chakagadzirwa chinogona kuchekwa ne beta-galactosidase, zvichikonzera kuburitswa kwechinhu chine ruvara rweyero. Hukuru hwe hydrolysis ye substrate hunogona kuverengerwa spectrophotometric nekuyera kunyudzwa kwechigadzirwa pahurefu hwakati. Izvi zvinobvumira vaongorori kuongorora mashandiro uye kinetics ye beta-galactosidase mumamiriro akasiyana-siyana, akadai sekudzidza mashandiro e enzyme, kuongorora ma enzyme inhibitors kana activators, kana kuongorora mhedzisiro yekuchinja kwezvinhu pamabasa e enzyme.

Odha kana Kumbira Mutengo

Ruzivo rweChigadzirwa

Matagi eChigadzirwa

Kushandiswa uye Mhedzisiro

Kuona mashandiro e beta-galactosidase: PNPG inowanzoshandiswa mukuyedza kuyera mashandiro e beta-galactosidase, enzyme inokonzeresa hydrolysis ye lactose kuita glucose ne galactose. Hydrolysis ye PNPG ne beta-galactosidase inoburitsa p-nitrophenol (pNP) molecule, iyo inogona kuonekwa ne spectrophotometric nekuda kweruvara rwayo rweyero.

Kuongororwa kwevanodzivirira ma enzyme nevanoactivator: PNPG inogona kushandiswa mukuongorora kwe high-throughput kuti ione makemikari anogadzirisa basa re beta-galactosidase. Nekuyera mwero wePNPG hydrolysis pamberi pemakemikari akasiyana ekuyedza, vaongorori vanogona kuona zvinodzivirira zvinoderedza basa re enzyme kana zvinovandudza basa re enzyme.

Kudzidza kinetics yeenzyme: Kubviswa kwePNPG ne beta-galactosidase kunotevera kinetics yeMichaelis-Menten, zvichibvumira vaongorori kuona ma parameter eenzyme akakosha akadai se maximum reaction velocity (Vmax) uye Michaelis constant (Km). Ruzivo urwu runobatsira mukunzwisisa hukama hwe enzyme ne catalytic efficiency.

Mashandisirwo eMolecular biology: Beta-galactosidase, iyo inocheka PNPG, inowanzoshandiswa sereporter gene mu molecular biology. PNPG substrate inowanzo shandiswa kuona nekuona kuratidzwa kwereporter gene, zvichipa nzira iri nyore uye inonzwisisika yekuongorora kuratidzwa kwemajini mumaitiro akasiyana-siyana ekuyedza.

Muenzaniso weChigadzirwa

Kurongedza Chigadzirwa:

Mamwe Mashoko:

Kuumbwa	C18H25NO13
Kuyedza	99%
Chitarisiko	Upfu chena
Nhamba yeCAS	4419-94-7
Kurongedza	Diki uye rakakura
Hupenyu hweSherufu	makore maviri
Kuchengetera	Chengeta munzvimbo inotonhorera uye yakaoma
Chitupa	ISO.