5,5-Dithiobis (2-nitrobenzoic acid) CAS:69-78-3

5,5-Dithiobis (2-nitrobenzoic acid), inozivikanwawo seDTNB kana kuti Ellman's reagent, ikemikari inoshandiswa zvakanyanya muongororo yemakemikari nemishonga. Ihupfu hweyero, hwakaita sekristalo hunonyungudika zvakanyanya mumvura uye mune zvinonyungudutsa zvakasikwa.

DTNB inonyanya kushandiswa kuyera huwandu hwemapoka e-sulfhydryl (-SH) mumapuroteni nemapeptide. Musanganiswa uyu unoshanda pamwe chete nemathiols, zvichikonzera kuumbwa kwemusanganiswa we disulfide uye kuburitsa 2-nitro-5-thiobenzoate, ine ruvara rweyero runogona kuyerwa ne spectrophotometric. Nekuyera kusimba kweruvara rweyero, zvinokwanisika kufungidzira huwandu hwemathiols mumuenzaniso. Iyi nzira inowanzoshandiswa kudzidza kudyidzana kwemapuroteni nemapuroteni, kupeta mapuroteni, uye enzyme reactions dzinosanganisira mapoka ethiol.

Pamusoro pekuyera thiol, DTNB yakashandiswawo mune zvimwe zvinoshandiswa, zvakaita sekuongorora mamiriro emasero e redox, kudzidza kugadzikana kwemakemikari esimbi nesarufa, uye kuyera mashandiro ema enzyme akasiyana-siyana.

Odha kana Kumbira Mutengo

Ruzivo rweChigadzirwa

Matagi eChigadzirwa

Kushandiswa uye Mhedzisiro

Kuyera kweThiol: DTNB inowanzoshandiswa kuongorora huwandu hwethiols mumapuroteni. Ruzivo urwu rwakakosha pakudzidza kudyidzana kwemapuroteni nemapuroteni, kupetwa kwemapuroteni, uye maitiro eenzymes ane chekuita nemapoka ethiol. Mhinduro yemavara eDTNB inobvumira kuyerwa kwethiols kuburikidza nekuyera kwespectrophotometric.

Kuongororwa kweRedox State: DTNB yakashandiswa kuongorora mamiriro eredox mukati memasero, nyama, kana sampuli dzebhayoloji. Nekuyera shanduko muhuwandu hwethiol, vaongorori vanogona kuwana ruzivo nezve oxidative stress, antioxidant activity, uye redox signaling pathways.

Kuyedzwa kweEnzyme: Kushanda kwakasiyana kweDTNB nethiols kunoita kuti ive chishandiso chakakosha chekuongorora maenzyme. Maenzyme mazhinji, akadai seglutathione-S-transferases, cystathionine-γ-lyase, uye thioredoxin reductase, anoenderana nethiol uye anogona kuverengerwa kana kuyedzwa uchishandisa DTNB.

Kuongororwa Kwekugadzikana KwemaCompounds eMetal-Sulfur: DTNB inoshandiswa kuongorora kugadzikana uye reactivity ye metal-sulfur complexes uye metalloproteins. Kompositi iyi inogona kuona uye kuverenga kuvapo kwe thiols dzinobatanidzwa mukucheneswa kwemaion esimbi, ichipa ruzivo rwakakosha nezvekudyidzana kwe metal-sulfur uye coordination chemistry.

Kurongedza Chigadzirwa:

Mamwe Mashoko:

Kuumbwa	C14H8N2O8S2
Kuyedza	99%
Chitarisiko	Yero yakasimba
Nhamba yeCAS	69-78-3
Kurongedza	Diki uye rakakura
Hupenyu hweSherufu	makore maviri
Kuchengetera	Chengeta munzvimbo inotonhorera uye yakaoma
Chitupa	ISO.