Avidin CAS:1405-69-2 Mutengo wemugadziri

Kucheneswa kweMapuroteni: Avidin inogona kuvharwa nechinhu chakasimba, senge chromatography resin kana magnetic beads, uye inoshandiswa kubata mapuroteni ane biotinylated kana nucleic acids. Iyi nzira inozivikanwa seavidin affinity chromatography, uye inogonesa kucheneswa kwemamorekuru chaiwo nenzira chaiyo.

Mapurobe Ane Biotinylated: Avidin inowanzobatanidzwa nema enzymes, fluorophores, kana mamwe mavara uye inoshandiswa senzira yekuona muongororo dzakasiyana-siyana. Mapurobe ane biotinylated, akadai sema antibodies kana nucleic acid probes, anogona kuonekwa nekusunga kumamorekuru ekuona avidin-linked. Iyi nzira inowanzoshandiswa muELISA, immunohistochemistry, uye nucleic acid hybridization assays.

Kuendeswa Kwemishonga Yakanangana: Avidin inogona kushandiswa semorekuru inotakura mishonga kana mishonga yekurapa kunyanya kumasero kana tishu dzinoburitsa biotin receptor. Nekubatanidza biotin nemishonga kana nanoparticles uye kuzvibatanidza neavidin, masisitimu ekutumira mishonga akanangana anogona kugadzirwa.

Matekiniki eMolecular Biology: Masisitimu akavakirwa paAvidin anoshandiswa zvakanyanya mukutsvagisa kweMolecular biology. Semuenzaniso, streptavidin, iyo ine hukama hwakafanana nebiotin seavidin, inoshandiswa muhunyanzvi hwakadai seDNA sequencing, PCR, uye in situ hybridization, uko inosanganiswa ne biotinylated probes kana primers.

Kufungidzira uye Kuongorora: Avidin inogona kunyorwa ne radioisotopes, dyes dze fluorescent, kana nanoparticles, zvichiita kuti ive chishandiso chakakosha muhunyanzvi hwekufungidzira senge positron emission tomography (PET), fluorescence microscopy, uye magnetic resonance imaging (MRI). Pamusoro pezvo, masisitimu akavakirwa pa avidin anoshandiswa mukuyedza kuongorora, senge immunoassays, kuona kuvapo kwe biomarkers chaiyo.