BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine munyu CAS:6578-06-9

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine munyu ikemikari inoshandiswa zvakanyanya muongororo ye molecular biology ne biochemistry. Inowanzoshandiswa mu enzyme-linked immunosorbent assays (ELISA) uye nzira dzekuisa ma protein labeling.

Musanganiswa uyu imhando yealkaline phosphatase, enzyme inoshandiswa zvakanyanya mu molecular biology pakuona kuvapo kwemamorekuru kana mapuroteni kuburikidza ne colorimetric kana chemiluminescent reactions.

Kana enzyme inonzi alkaline phosphatase iripo, inobvisa mapoka ephosphate kubva mumunyu we5-Bromo-4-chloro-3-indolyl phosphate p-toluidine, zvichikonzera kuumbwa kwedhayi rebhuruu kana repepuru. Kuchinja kwemavara uku kunogona kuonekwa nyore nyore uye kuverengwa, zvichiita kuti kuyerwe kwekushanda kweenzyme kana kuvapo kwemamorekuru akananga.

Odha kana Kumbira Mutengo

Ruzivo rweChigadzirwa

Matagi eChigadzirwa

Kushandiswa uye Mhedzisiro

Kubvisa mabhakitiriya eWestern: BCIP inoshandiswa kuona nekuona mapuroteni anotamisirwa pamembrane mushure me gel electrophoresis. Ma antibodies echipiri eAlkaline phosphatase-conjugated anoshandiswa kusunga ma antibodies ekutanga, uye kusanganiswa kweBCIP kunotevera kunoita kuti pave neblue precipitate panzvimbo yema protein bands.

Immunohistochemistry: BCIP inoshandiswa muhunyanzvi hwekuona mapuroteni chaiwo mumatishu. Ma antibodies echipiri eAlkaline phosphatase-conjugated anoshandiswa kusunga ma antibodies ekutanga, uye kurapwa kweBCIP kunoita kuti ruvara rwebhuruu ruonekwe kana enzyme iripo, zvichiratidza kuti mapuroteni ari mumatishu acho aripo.

Kusanganisa zvinhu munzvimbo imwe chete: BCIP inoshandiswawo pakuona nucleic acid sequences mumatishu kana masero akagadzika. Mahybridization probes akanyorwa nealkaline phosphatase, uye kana BCIP yawedzerwa, kuvapo kwetarget mRNA kana DNA kunoratidzwa nekukura kweruvara rwebhuruu.

Kurongedza Chigadzirwa:

Mamwe Mashoko:

Kuumbwa	C8H6BrClNO4P.C7H9N
Kuyedza	99%
Chitarisiko	Upfu chena
Nhamba yeCAS	6578-06-9
Kurongedza	Diki uye rakakura
Hupenyu hweSherufu	makore maviri
Kuchengetera	Chengeta munzvimbo inotonhorera uye yakaoma
Chitupa	ISO.