DEPC CAS:1609-47-8 Mutengo wemugadziri

Diethyl pyrocarbonate (DEPC) ikemikari inoshandiswa zvakanyanya mumarabhoritari ebiology yemolecular. Imvura yakajeka uye isina ruvara inorwisa zvakanyanya manyuko enucleophilic akadai seamines nethiols. DEPC inonyanya kushandiswa kudzima maRNases (ribonucleases), maenzyme anogona kukanganisa RNA, nekushandura nzvimbo dzawo dzinoshanda. Inoita izvi nekuita nema histidine residues mu enzyme, ayo akakosha pakushanda kwayo. Izvi zvinoita kuti DEPC ive chishandiso chakakosha mukuyedza kwakabatana neRNA uye inovimbisa kuchengetedzwa kweRNA. Pamusoro pehunhu hwayo hwekudzima maRNase, DEPC inogona zvakare kugadzirisa mamwe ma biomolecule, akadai semapuroteni neDNA, nekuda kwekuita kwayo nema nucleophilic groups. Kungwarira kunofanirwa kutorwa pakubata DEPC, sezvo ine chepfu uye inogona kuchinja.

Odha kana Kumbira Mutengo

Ruzivo rweChigadzirwa

Matagi eChigadzirwa

Kushandiswa uye Mhedzisiro

Kusashanda kweRNases: Chinangwa chikuru cheDEPC ndechekusashanda kweRNases, ayo ari ma enzyme anogona kukanganisa RNA. DEPC inobatana nehistidine residues muRNases, ichichinja nzvimbo dzayo dzinoshanda uye ichidziita kuti dzisashande. Izvi zvinovimbisa kuchengetedzwa kweRNA integrity panguva yekuedza kwakadai sekupatsanurwa kweRNA, reverse transcription, uye RNA sequencing.

Kuchengetedzwa kwemasampuli eRNA: Kurapa kweDEPC kunowanzo shandiswa pamidziyo yemurabhoritari, senge machubhu e microcentrifuge, mapipette tips, nemvura inoshandiswa mukuyedza kweRNA. Izvi zvinobatsira kudzivirira chero maRNases asara anogona kunge aripo uye kudzivirira kuparara kweRNA panguva yekubata nekuchengetedza sampuro.

Kugadzirira reagent isina RNAse: DEPC inogona kushandiswa kurapa mhinduro kana reagents dzinofanira kunge dzisina RNAse, dzakadai se buffers, Tris-EDTA (TE) solutions, kana mvura inoshandiswa mukuyedza kwe molecular biology. Mhinduro idzi dzakarapwa dzinogona kushandiswa zvakachengeteka kuderedza kuora kweRNA.

Kugadziriswa kweDNA: DEPC inogonawo kuita nemaamino groups mumamorekuru eDNA, zvichikonzera kugadziriswa kweDNA bases necarbethoxylated. Izvi zvinogona kushandiswa mune mamwe mashandisirwo, akadai seDNA sequencing reactions kana kugadzirisa DNA kuti iwane zvidzidzo zve crosslinking.

Kurongedza Chigadzirwa:

Mamwe Mashoko:

Kuumbwa	C6H10O5
Kuyedza	99%
Chitarisiko	Mvura isina ruvara
Nhamba yeCAS	1609-47-8
Kurongedza	Diki uye rakakura
Hupenyu hweSherufu	makore maviri
Kuchengetera	Chengeta munzvimbo inotonhorera uye yakaoma
Chitupa	ISO.