DNase I, kubva kuBovine Pancreas CAS:9003-98-9

DNase I, inotorwa kubva kupancreas yemombe, ienzyme inonyanya kushandisa hydrolyze mamorekuru eDNA. Inocheka ma phosphodiester bonds ari mumusana weDNA, zvichikonzera kuparara kweDNA kuita zvidimbu zvidiki. DNase I inoshandiswa zvakanyanya mukutsvagisa mamorekuru ebiology, kunyanya mukushandiswa kunosanganisira kupatsanurwa kweDNA, kucheneswa, uye kugadziriswa. Inoshandiswa kubvisa kusvibiswa kweDNA kubva muRNA samples, kuderedza DNA muma protein-DNA complexes, uye kupatsanura DNA mukuyedza kwakasiyana-siyana kwakadai seDNA footprinting uye chromatin analysis. DNase I inowanawo mashandisirwo mukuongorora kwekurapa, uko inoshandiswa kuona nekuenzanisa DNA isina masero muropa rinotenderera, pamwe nesainzi ye forensic pakuongorora humbowo hweDNA.

Odha kana Kumbira Mutengo

Ruzivo rweChigadzirwa

Matagi eChigadzirwa

Kushandiswa uye Mhedzisiro

DNase I, inobva kupancreas yemombe, ienzyme inocheka mamorekuru eDNA nekusanganisa phosphodiester bonds mumusana weDNA. Mhedzisiro huru yeDNase I ndeyekuora nekupatsanurwa kweDNA.

Kushandiswa kukuru kweDNase I kuri muongororo yebiology yemamorekuru. Inowanzoshandiswa mukuyedza kwakasiyana-siyana kupatsanura nekuchenesa DNA, kubvisa kusvibiswa kweDNA kubva muRNA samples, uye kugadzirisa DNA. DNase I inonyanya kushanda mukuderedza DNA mumaprotein-DNA complexes, zvichibvumira vaongorori kudzidza kudyidzana kweprotein-DNA kana kubvisa mapuroteni akabatana neDNA.

Pamusoro pezvo, DNase I inoshandiswa kakawanda mukuongorora DNA footprinting, iyo inobatsira kuona nzvimbo dzeDNA dzakasungwa nemapuroteni chaiwo. Nekurapa sample yeDNA neDNase I uye kuongorora zvidimbu zveDNA zvinobva, vaongorori vanogona kuona nzvimbo dzinosunga mapuroteni eDNA.

DNase I inoshandiswawo mukuongorora chromatin, uko inobatsira mukugaya chromatin (DNA nemapuroteni akabatana nayo) kuti idzidze chimiro chayo nekurongeka kwayo. Nekugaya chromatin neDNase I, vaongorori vanogona kuona nzvimbo dziri nyore kusvikika uye dzinonzwa kupatsanuka, zvichipa ruzivo rwekudzora majini uye chimiro chechromatin.

Uyezve, DNase I inoshandiswa mukuongorora zvirwere, kunyanya pakuona nekuongorora DNA isina masero muropa rinotenderera. Nekushandisa DNase I kugaya DNA isingatenderere, zvinokwanisika kuongorora nekuona marongerwo chaiwo eDNA ane chekuita nezvirwere kana kusarongeka kwemajini.

Kurongedza Chigadzirwa:

Mamwe Mashoko:

Kuumbwa	NA
Kuyedza	99%
Chitarisiko	Upfu chena
Nhamba yeCAS	9003-98-9
Kurongedza	Diki uye rakakura
Hupenyu hweSherufu	makore maviri
Kuchengetera	Chengeta munzvimbo inotonhorera uye yakaoma
Chitupa	ISO.