Ethidium Bromide CAS:1239-45-8

Ethidium bromide (EtBr) idhayi rinoshandiswa mu fluorescent biology uye biochemistry pakuona nucleic acids, kunyanya DNA, mu agarose gel electrophoresis. Ine hukama hwakakura neDNA uye, kana ikasangana nechiedza che ultraviolet (UV), inopenya uye inoburitsa ruvara rutsvuku-orenji. Izvi zvinoita kuti vaongorori vakwanise kuona nekuongorora zvidimbu zveDNA zvakakamurwa nehukuru mu gel matrix.

EtBr inopindirana pakati pezvikamu zveDNA neRNA, zvichikonzera shanduko muhunhu hwayo hwekupenya. Inowanzo wedzerwa mumajeli eagarose kana kusanganiswa nemasamples eDNA isati yaitwa electrophoresis. Kana jeli iri pachiedza cheUV, mabhendi eDNA ane EtBr anoonekwa semabhandi eorenji akajeka achipesana nerima, zvichiita kuti zvive nyore kuona nekuongorora zvidimbu zveDNA.

Odha kana Kumbira Mutengo

Ruzivo rweChigadzirwa

Matagi eChigadzirwa

Kushandiswa uye Mhedzisiro

Kuona DNA/RNA: EtBr inonyanya kushandiswa kufungidzira DNA neRNA mu agarose gel electrophoresis. Inobatanidza pakati pema base pairs ema nucleic acid molecules, zvichiita kuti asvibiswe kana aiswa muchiedza cheUV. Izvi zvinobvumira vaongorori kufungidzira nekuongorora kupatsanurwa kwezvikamu zveDNA kana RNA zvichienderana nehukuru hwazvo kana kutevedzana kwazvo.

Kusvibisa DNA: EtBr inogona kushandiswa kusvibisa DNA munzira dzakasiyana-siyana dzekuedza. Inogona kuwedzerwa kumasamples eDNA, akadai semaplasmid akacheneswa kana zvigadzirwa zvePCR, kuti ionekwe kuvapo kwavo uye huwandu hwavo. DNA yakacheneswa inogona kuratidzwa uchishandisa chiedza cheUV kana sisitimu yekufungidzira gel.

Kuyera DNA: EtBr inogona kushandiswa pakuenzanisa kuwanda kweDNA. Nekuenzanisa kusimba kweEtBr-stained DNA bands mu agarose gel nemazinga anozivikanwa ekuwanda kweDNA, huwandu hunofungidzirwa hweDNA mumuenzaniso hunogona kuonekwa.

Microscopy yeFluorescent: EtBr inogona kushandiswa mu fluorescence microscopy kuti iparadze uye ione DNA mumasero mapenyu kana kuti nyama. Izvi zvinobvumira vaongorori kudzidza nzvimbo yeDNA uye mashandiro ayo mumasero kana kuona kupatsanurwa kweDNA mumasero eapoptosis.

Kurongedza Chigadzirwa:

Mamwe Mashoko:

Kuumbwa	C21H20BrN3
Kuyedza	99%
Chitarisiko	Upfu hwakatsvuka kusvika hwepepuru yakasviba
Nhamba yeCAS	1239-45-8
Kurongedza	Diki uye rakakura
Hupenyu hweSherufu	makore maviri
Kuchengetera	Chengeta munzvimbo inotonhorera uye yakaoma
Chitupa	ISO.