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N,N-Bis(2-hydroxyethyl) -2-aminoethanesulfonic acid sodium munyu CAS:66992-27-6
N,N-Bis(2-hydroxyethyl) -2-aminoethanesulfonic acid sodium munyu, inozivikanwawo seHEPES sodium munyu, ikemikari inosanganiswa inowanzoshandiswa se pH buffering agent mubiological and chemical laboratories.Inobatsira kuchengetedza yakagadzikana pH renji, ichiita kuti ive yakakodzera kune mashandisirwo akadai sesero tsika, enzyme assays, zvidzidzo zveprotein, electrophoresis, uye kugadzirwa kwemishonga.HEPES sodium munyu inovimbisa mamiriro akakwana ezvipenyu uye inosimudzira huchokwadi uye kuvimbika kwemhedzisiro yekuedza.
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S-Butyrylthiocholine iodide CAS:1866-16-6
S-Butyrylthiocholine iodide ikemikari yemakemikari inowanzoshandiswa mu biochemical uye enzymatic assays.Iyo substrate ye enzyme butyrylcholinesterase (BChE) uye inoshandiswa kuyera basa rayo.
Apo S-Butyrylthiocholine iodide inogadzirwa ne hydrolyzed neBChE, inobudisa thiocholine uye butyric acid sezvigadzirwa.Kuburitswa kwetiocholine kunogona kuyerwa uchishandisa spectrophotometric kana fluorometric assay, zvichibvumira quantification yeBChE chiitiko.
S-Butyrylthiocholine iodide inowanzoshandiswa mumakiriniki uye zvirongwa zvekutsvaga kuongorora basa reBChE mumasampuli akadai seplasma yeropa kana matishu.Inogona kushandiswa kuongorora enzymatic basa reBChE uye rinogona kuita basa rayo mune dzakasiyana siyana biological process, pamwe nekuongorora uye kutarisa kwemamwe mamiriro ekurapa.
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ABTS (2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium munyu) CAS:30931-67-0
Diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate), inowanzonzi ABTS, inowanzoshandiswa chromogenic substrate mu biochemical assays, kunyanya mumunda we enzymology.Mushonga wekugadzira unoshandiswa kuyera basa rema enzymes akasiyana siyana, kusanganisira peroxidases uye oxidases.
ABTS haina ruvara muchimiro chayo chine oxidized asi inoshanduka yeblue-green kana yaiswa oxidized neenzyme pamberi pehydrogen peroxide kana molecular oxygen.Uku kushanduka kwemavara kunokonzerwa nekuumbwa kwekasikisi yakakura, iyo inotora chiedza mumagetsi anooneka.
Kuita pakati peABTS ne enzyme inoburitsa chigadzirwa chine ruvara chinogona kuyerwa spectrophotometrically.Kusimba kweruvara kwakanyatso kuenderana nebasa re enzymatic, zvichibvumira vaongorori kuti vaongorore zvakanyanya enzyme kinetics, enzyme inhibition, kana enzyme-substrate kupindirana.
ABTS ine huwandu hwakawanda hwekushandisa munzvimbo dzakasiyana siyana, kusanganisira kuongororwa kwekiriniki, tsvakiridzo yemishonga, uye sainzi yechikafu.Iyo ine hanya zvakanyanya uye inopa yakafara simba renji, ichiita iyo yakakurumbira sarudzo kune akawanda biochemical assays.
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4-NITROPHENYLΑ-D-MALTOHEXAOSIDE CAS:74173-30-1
4-Nitrophenyl α-D-maltohexaoside inhengo yekirasi ye-α-glycosidic linkages.Inobva kune maltose, iyo disaccharide inoumbwa nezvikamu zviviri zveglucose.Muchikamu ichi, boka rehydroxyl rekutanga glucose unit rinotsiviwa ne nitrophenyl moiety.
Mushonga uyu unowanzo shandiswa se substrate mune enzymatic assays yekudzidza mashandiro ema enzymes akasiyana, kunyanya ayo anobatanidzwa mucarbohydrate metabolism.Boka renitrophenyl rinobvumira kuti zvionekwe zviri nyore uye chiyero chemaitiro enzymatic nekuyera kunyura kana fluorescence yechigadzirwa chakatsemuka.
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PIPES CAS:5625-37-6 Mutengo wemugadziri
PIPES (piperazine-1,4-bisethanesulfonic acid) is zwitterionic buffering compound inowanzoshandiswa mukutsvakurudza kwebiological uye biochemical.Iyo inoshanda pH buffer ine hunyanzvi hwekuchengetedza yakagadzikana pH mamiriro mupH ye6.1 kusvika 7.5.PIPES ine kukanganiswa kudiki nemabiomolecules uye inokodzera tembiricha-inotsamira assays.Inowanzoshandiswa mune gel electrophoresis maitiro uye kugadzirwa kwemishonga semugadziri wekugadzikana.Pakazere, PIPES inosiyana-siyana uye inoshandiswa zvakanyanya mumichina yekuedzwa kwakasiyana-siyana.
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3,3′,5,5′-Tetramethylbenzidine CAS:207738-08-7
3,3',5,5'-Tetramethylbenzidine, inozivikanwawo seTMB, ikemikari inowanzoshandiswa sechromogenic substrate mu enzyme-linked immunosorbent assays (ELISA) uye mamwe maitiro ezvinyorwa zvebiochemical.Inowanzoshandiswa kuona uye kuverenga kuvepo kwema enzymes akadai sehorseradish peroxidase (HRP) mune dzakasiyana siyana biological samples.TMB inochinja ruvara kubva paruvara rusina ruvara kuenda rwebhuruu kana maenzayimu aya aripo.Zvadaro, maitiro anogona kumiswa nekuwedzera asidhi inoshandura ruvara rwebhuruu kune yekupedzisira yeyero ruvara.Kusimba kweyero ruvara kunoenderana nehuwandu hwe enzyme iripo, ichibvumira quantification.
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APS-5 CAS:193884-53-6 Mutengo wemugadziri
(4-Chlorophenyl) thio-methanol 1- (dihydrogen phosphate) disodium munyu (1: 2) ikemikari yemakemikari inowanikwa mukirasi yeacridine derivatives.Inosanganisira 10-methylacridine ring system ine thioether boka rinosungirirwa kune 4-chlorophenyl nzvimbo.Mushonga wacho unewo boka remethanol uye mapoka maviri efosfate ayo akadzivirirwa zvishoma ne sodium ions.
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5-Bromo-4-chloro-3-indolyl phosphate disodium munyu CAS:102185-33-1
5-Bromo-4-chloro-3-indolyl phosphate disodium munyu (BCIP) ikemikari inosanganiswa inowanzoshandiswa mumamolecular biology uye biochemistry applications.Iyo chromogenic substrate yealkaline phosphatase enzymes.
BCIP inowanzoshandiswa pamwe chete ne nitroblue tetrazolium (NBT) se substrate yekuona alkaline phosphatase chiitiko.Kana BCIP iine dephosphorylated nealkaline phosphatase, mafomu eblue precipitate mafomu, achibvumira kuratidzwa kwekuvapo kwe enzyme kana chiitiko.
Mushonga uyu unonyanya kubatsira mumashandisirwo akadai se immunohistochemistry, in situ hybridization, uye enzyme-yakabatana immunosorbent assays (ELISAs) kuona kuvepo kana nharaunda yeakananga biomolecules kana nucleic acids.Iyo yebhuruu precipitate yakaumbwa neBCIP inopa chiratidzo chinooneka chinobatsira mukuzivikanwa uye kuongororwa kwemamorekuru anonangwa mumasampuli ekuyedza.
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Sodium Munyu munyu CAS:139-41-3 Mutengo wemugadziri
N,N-Bis(2-hydroxyethyl) glycine sodium munyu ikemikari inoshandiswa seyakavharisa mumhando dzakasiyana dzebiochemical uye biophysical application.Inobatsira kuchengetedza yakagadzikana pH nhanho mumamiriro ekuyedza, zvichiita kuti ishande muzvidzidzo zve enzyme, tsvakiridzo yeprotein, sero tsika, uye Western blotting matekiniki.
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4-Aminophthalhydrazide AMPPD CAS:3682-14-2
4-Aminophthalhydrazide, inozivikanwawo se 4-APhH, ikemikari yemakemikari ine molecular formula C8H8N2O.Iyo ndeyekirasi ye hydrazide makomisheni uye inotorwa kubva kuphthalic acid.
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N-Acetyl-L-cysteine CAS:616-91-1
N-Acetyl-L-cysteine (NAC) imhando yakagadziridzwa yeamino acid cysteine.Inopa sosi yecysteine uye inogona kushandurwa nyore nyore kuita tripeptide glutathione, ine simba antioxidant mumuviri.NAC inozivikanwa neayo antioxidant uye mucolytic zvivakwa, zvichiita kuti ibatsire mune dzakasiyana siyana hutano maapplication.
Se antioxidant, NAC inobatsira kuchengetedza maseru kubva mukukuvadzwa kunokonzerwa nemahara radicals, reactive oxygen marudzi, uye chepfu.Iyo zvakare inotsigira glutathione synthesis, iyo inoita basa rakakosha mukuita kwemuviri detoxification maitiro uye kuchengetedza ine hutano immune system.
NAC yakadzidzwa nezve mabhenefiti ayo muhutano hwekufema, kunyanya kune vanhu vane mamiriro akaita senge chronic bronchitis, COPD, uye cystic fibrosis.Inowanzoshandiswa se expectorant kubatsira mutete uye kusunungura mucus, zvichiita kuti zvive nyore kubvisa mhepo.
Uyezve, NAC yakaratidza chivimbiso mukutsigira hutano hwechiropa nekubatsira mukubviswa kwezvinhu zvine chepfu, senge acetaminophen, inogadzirisa marwadzo.Inogonawo kuva nemigumisiro yekudzivirira pakukuvara kwechiropa kunokonzerwa nekunwa doro.
Pamusoro peiyo antioxidant uye yekufema kutsigira zvivakwa, NAC yakaongororwa nekuda kwezvainogoneka muhutano hwepfungwa.Imwe tsvakurudzo inoratidza kuti inogona kuva nemigumisiro yakanaka pakusagadzikana kwepfungwa, zvakadai sekuora mwoyo uye obsessive-compulsive disorder (OCD).
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Acetylthiocholine iodide CAS:1866-15-5
Acetylthiocholine iodide ikemikari yemakemikari inowanzoshandiswa se substrate mukuedza kwe enzyme kuyera basa re enzyme acetylcholinesterase (AChE).AChE is enzyme iyo hydrolyzes neurotransmitter acetylcholine, nhanho yakakosha mukugumisa kutapurirana kwechiratidzo pakati pemasero etsinga.
Kana acetylthiocholine iodide inoshandiswa neAChE, boka reacetyl rinobviswa, zvichiita kuti pave nekugadzirwa kweiyo thiocholine uye acetate ions.Thiocholine inobva yadaira nechinhu chisina ruvara chinonzi DTNB (5,5′-dithiobis(2-nitrobenzoic acid)) kugadzira komboni ine ruvara rweyero inonzi 5-thio-2-nitrobenzoate, iyo inogona kuyerwa spectrophotometrically.Chiyero chekukura kwemavara chakanyatso kuenderana nebasa reAChE mumuenzaniso.
