5-Bromo-4-chloro-3-indolyl phosphate disodium salt CAS:102185-33-1 Mufananidzo Unonyanya Kuonekwa

5-Bromo-4-chloro-3-indolyl phosphate disodium munyu CAS:102185-33-1

5-Bromo-4-chloro-3-indolyl phosphate disodium salt (BCIP) ikemikari inoshandiswa zvakanyanya mu molecular biology uye biochemistry. I chromogenic substrate ye alkaline phosphatase enzymes.

BCIP inowanzoshandiswa pamwe chete ne nitroblue tetrazolium (NBT) se substrate yekuona mashandiro ealkaline phosphatase. Kana BCIP yabviswa phosphorylated ne alkaline phosphatase, blue precipitate inoumbwa, zvichibvumira kuona kuvapo kana kushanda kwe enzyme.

Musanganiswa uyu unonyanya kubatsira mukushandiswa kwakadai se immunohistochemistry, in situ hybridization, uye enzyme-linked immunosorbent assays (ELISAs) kuti uone kuvapo kana kuti nzvimbo ye biomolecules chaiyo kana nucleic acids. Blue precipitate inoumbwa neBCIP inopa chiratidzo chinooneka chinobatsira mukuziva uye kuongorora mamorekuru anotarisirwa mumienzaniso yekuedza.

Odha kana Kumbira Mutengo

Ruzivo rweChigadzirwa

Matagi eChigadzirwa

Kushandiswa uye Mhedzisiro

Immunohistochemistry (IHC): BCIP inowanzoshandiswa muIHC assays kuti ione kuvapo kana kuti nzvimbo yemaantigen chaiwo mumienzaniso yenyama. Kupenya kwebhuruu kunogadzirwa neBCIP kunobvumira kuzivikanwa kwenzvimbo dzeantigen uye kunogona kushandiswa pakuongorora kwemhando yepamusoro kana kuti kwehafu-huwandu.

Kusanganiswa kwemukati memuviri (ISH): BCIP inowanzoshandiswa muhunyanzvi hweISH kuona nekuwana nzvimbo dzakatarwa dze nucleic acid, dzakadai se mRNA kana DNA. Kupenya kwebhuruu kunoumbwa ne BCIP kunoratidza kuvapo kwezvikamu zvakatarwa uye kunobatsira mukuona kupararira kwazvo mukati memasero kana matishu.

Kuyedzwa kweEnzyme-linked immunosorbent assays (ELISAs): BCIP dzimwe nguva inoshandiswa muELISAs kuona kuvapo kana kuwanda kwema biomolecules chaiwo, akadai semapuroteni kana ma antibodies. Nekubatanidza BCIP ne enzyme label yakakodzera uye kushandisa alkaline phosphatase kuti iite kuti reaction iitike, ruvara rwebhuruu rwunogadzirwa runogona kuratidza kuvapo uye kuwanda kwema biomolecule anodiwa.

Kurongedza Chigadzirwa:

Mamwe Mashoko:

Kuumbwa	C8H7BrClNNaO4P
Kuyedza	99%
Chitarisiko	Upfu chena
Nhamba yeCAS	102185-33-1
Kurongedza	Diki uye rakakura
Hupenyu hweSherufu	makore maviri
Kuchengetera	Chengeta munzvimbo inotonhorera uye yakaoma
Chitupa	ISO.