Fluorescein Isothiocyante CAS:3326-32-7

Hunhu hweFluorescence: FITC ine goho rakakura uye inoburitsa fluorescence yegirini yakajeka kana yamutswa pa 488 nm. Hunhu hwayo hweFluorescence hunoita kuti ikodzere kuonekwa uchishandisa nzira dzakasiyana-siyana dzekufungidzira, dzakadai se fluorescence microscopy, flow cytometry, uye fluorescence spectroscopy.

Kusungana kweCovalent: FITC ine boka re isothiocyanate rinoibvumira kubatana pamwe chete nemapoka emaamino ari mumapuroteni, ma antibodies, uye mamwe ma biomolecule. Kusungana uku kwecovalent kunoita kuti mamorekuru anotarisirwa anyatso nyorwa, zvichiita kuti aonekwe uye aonekwe.

Immunofluorescence: Ma antibodies ane label yeFITC anoshandiswa zvakanyanya mukuyedza immunofluorescence kuona maantigen chaiwo kana mapuroteni mumasero nematishu. Kupenya kwegirini kwakasvibira kunoburitswa neFITC kunoita kuti vaongorori vakwanise kuona uye kuona kupararira kwemamorekuru ari kudiwa mukati memasampuli ehupenyu.

Flow cytometry: Ma antibodies ane label yeFITC kana mamwe ma biomolecules akabatana ne fluorochrome anoshandiswa zvakanyanya mu flow cytometry kuongorora nekugadzira masero zvichienderana nezviratidzo zvawo kana zvikamu zvemukati memasero. FITC inoenderana ne 488 nm laser inowanzoshandiswa mu flow cytometry instruments.

Zvidzidzo zvekudyidzana kwemapuroteni: FITC inogona kushandiswa muzvidzidzo zvekudyidzana kwemapuroteni nemapuroteni kuti iongorore kubatana uye kudyidzana kwemapuroteni. Nekunyora mapuroteni chaiwo neFITC, vaongorori vanogona kuongorora kudyidzana kwavo vachishandisa matekiniki akadai se fluorescence resonance energy transfer (FRET) kana fluorescence polarization.

Kutevera mamorekuru emuviri: FITC inogona kushandiswa kutevedzera nekutevera mafambiro emamorekuru emuviri mukati memasero mapenyu kana matishu. Nekunyora mazita emamorekuru anodiwa neFITC, vaongorori vanogona kudzidza nzvimbo yavo, mafambiro avo, uye maitiro ekutengeserana munguva chaiyo vachishandisa fluorescence microscopy.

Mashandisirwo ekuongorora chirwere: Mapurobhu kana ma antibodies ane label yeFITC anoshandiswa mukuongorora chirwere ichi pakushandiswa kwakasiyana-siyana, kusanganisira kuona maantigen chaiwo, zvinhu zvinotapukira, kana biomarkers musampuli dzevarwere. Mapurobhu aya e fluorescent anopa kuongororwa kwakanyatsojeka uye kwakananga, zvichibvumira kuongororwa nekukurumidza uye nemazvo.