FLUORESCEIN MONO-BETA-D- GALACTOPYRANOSIDE CAS:102286-67-9

Fluorescein mono-beta-D-galactopyranoside, inozivikanwawo seFMG, inhengo yefluorescent iyo inowanzoshandiswa se substrate mune akasiyana biochemical uye cell biology kuyedza.Inotorwa kubva kumethyl-beta-D-galactopyranoside nekuibatanidza ne fluorescein molecule.FMG inoshandiswa zvakanyanya kudzidza basa re beta-galactosidase, enzyme inokonzera hydrolysis ye lactose mu galactose uye glucose.Nekushandisa FMG se substrate, vaongorori vanogona kutarisa enzymatic chiitiko che beta-galactosidase kuburikidza nechiyero che fluorescence emission.Iyo hydrolysis yeFMG ne beta-galactosidase inotungamirira pakusunungurwa kwefluorescein, zvichiita kuti kuwedzerwa kwechiratidzo che fluorescent chinogona kuverengwa.Ichi chikamu chinoshandiswawo kuongorora kuzivikanwa kwemakhahydrates nekubatana.FMG inogona kushandiswa seye molecular probe yekudzidza inosunga hukama hwelectins (mapuroteni anosunga zvakanyanya kumacarbohydrates) kune galactose-ine macarbohydrates.Iko kusungirirwa kweFMG-lectin complexes inogona kuonekwa uye kuverengwa zvichienderana nekuchinja kwefluorescence emission.Pakazere, FMG chishandiso chakasiyana-siyana mukudzidza basa re enzyme uye kucherechedzwa kwemakhahydrates, inopa nzira iri nyore uye inonzwisisika yekuyera fluorescence uye kuongorora aya maitiro ehupenyu.

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Fluorescein mono-beta-D-galactopyranoside (FMG) imorekuru rinowanzoshandiswa mukutsvagisa biology se substrate kuona kuvepo uye kuita kwebeta-galactosidase enzyme.FMG inotorwa kubva kune shuga lactose uye inosanganiswa ne fluorescein molecule.

Mhedzisiro huru yeFMG ndeyekuti yakanyatso hydrolyzed ne beta-galactosidase, enzyme inoputsa lactose kuita galactose uye glucose.Iyi enzymatic hydrolysis yeFMG inotungamira mukuburitswa kwefluorescein, iyo inoburitsa yakasimba fluorescence chiratidzo.

Iyo yekutanga kushandiswa kweFMG iri mukuona uye kuyera beta-galactosidase chiitiko mumasampuli akasiyana.Iyi enzyme inowanikwa mune zvakawanda zvipenyu, kusanganisira mabhakitiriya uye masero emammalian, uye basa rayo rinogona kuratidza maitiro akasiyana-siyana emasero uye nzira dzemagetsi.

Nekushandisa FMG se substrate, beta-galactosidase chiitiko chinogona kuyerwa nekutarisa fluorescence inoburitswa neyakasunungurwa fluorescein.Chiyero ichi chinogona kuitwa mumhando dzakasiyana dzekuyedza setups, kusanganisira in vitro assays uye live cell imaging zvidzidzo.

Uyezve, FMG inogona kushandiswa sechishandiso chekudzidza kugovera uye kuiswa kwebeta-galactosidase mukati memasero.Nekushandisa fluorescent microscopy matekiniki, vaongorori vanogona kuona iyo fluorescence inoburitswa neFMG pane hydrolysis, ichivabvumira kuti vatarise chiitiko chepakati uye chenguva chebeta-galactosidase.

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Composition	C26H22O10
Assay	99%
Chitarisiko	Upfu chena
CAS Nha.	102286-67-9
Packing	Zvidiki uye zvakawanda
Sherufu Hupenyu	2 years
Storage	Chengetedza munzvimbo inotonhorera uye yakaoma
Certification	ISO.