Gly-Pro p-nitroanilide p-toluenesulfonate munyu CAS:65096-46-0

Munyu weGly-Pro p-nitroanilide p-toluenesulfonate ikemikari inoshandiswa mukuyedza ma enzymes kuyera mashandiro ema proline-specific peptidases. Une dipeptide Gly-Pro yakabatana neboka re p-nitroanilide, iro rinogona kuburitswa nekushanda kwema proline-specific peptidases.

Mukuongorora ma enzyme, chinhu ichi chinowedzerwa mumuenzaniso une enzyme inodiwa. Enzyme iyi inobvisa chisungo pakati peglycine (Gly) neproline (Pro), ichiburitsa boka re p-nitroanilide. P-nitroanilide inoburitswa inogona kuyerwa ne spectrophotometric, zvichibvumira kuona mashandiro e enzyme.

Munyu wep-toluenesulfonate weGly-Pro p-nitroanilide unopa kugadzikana uye kunyungudika mumvura, zvichiita kuti zvikwanise kushandiswa mukuyedza ma enzymes..

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Kushandiswa uye Mhedzisiro

Mhedzisiro: Munyu weGly-Pro p-nitroanilide p-toluenesulfonate wakagadzirwa kuti uve substrate yemapeptidases chaiwo eproline. Maenzyme aya anoparadzanisa chisungo chepeptide pakati peglycine (Gly) neproline (Pro) residues, zvichiburitsa p-nitroanilide (pNA) sechigadzirwa. Molekyuru yepNA inonwisa chiedza pahurefu hwakati, zvichibvumira kuyerwa kwepNA yakaburitswa uye kufungidzira mashandiro eenzyme.

Mashandisirwo: Munyu weGly-Pro p-nitroanilide p-toluenesulfonate unoshandiswa munzvimbo dzakasiyana-siyana dzekutsvagisa, kusanganisira biochemistry, enzymology, uye kuwanikwa kwemishonga, mukushandiswa kunotevera:

Kuyedza mashandiro ema enzyme: Inoshandiswa zvakanyanya kuyera mashandiro ema proline-specific peptidases, kusanganisira dipeptidyl peptidases (DPPs) uye prolyl endopeptidases (PEPs).

Mishonga inodzivirira ma enzyme: Munyu weGly-Pro p-nitroanilide p-toluenesulfonate unogona kushandiswa kuongorora kugona kwemishonga kana mishonga pa proline-specific peptidase activity. Kuderera kwekuburitswa kwepNA kunoratidza kuvapo kwemushonga unodzivirira.

Zvidzidzo zveEnzyme kinetics: Nekusiyanisa huwandu hweGly-Pro p-nitroanilide p-toluenesulfonate salt uye kuyera mwero wekuburitswa kwepNA, ma enzyme kinetics parameters akadai seKm (Michaelis-Menten constant) uye Vmax (maximum velocity) anogona kuwanikwa.

Mashandisirwo ekuongorora: Kushanda kwemapeptidases chaiwo eproline kunogona kubatsira mukuongororwa kwezvirwere zvakasiyana-siyana, zvakaita sehutachiona nekenza. Munyu weGly-Pro p-nitroanilide p-toluenesulfonate unogona kushandiswa sechishandiso chekuongorora huwandu hwemaenzyme aya mumienzaniso yevarwere.

Kurongedza Chigadzirwa:

Mamwe Mashoko:

Kuumbwa	C20H24N4O7S
Kuyedza	99%
Chitarisiko	Upfu chena
Nhamba yeCAS	65096-46-0
Kurongedza	Diki uye rakakura
Hupenyu hweSherufu	makore maviri
Kuchengetera	Chengeta munzvimbo inotonhorera uye yakaoma
Chitupa	ISO.