HEPES-Na CAS:75277-39-3 Mutengo Wemugadziri

Buffering agent: HEPES sodium munyu inowanzoshandiswa seyakavharisa muyedzo yebiological uye biochemical.Inobatsira kuchengetedza pH yakagadzikana, kunyanya muhutano hwehupenyu (pH 7.2-7.6).Iyo buffering kugona kwayo inoita kuti ive yakakosha mukuchengetedza mamiriro akakodzera eakasiyana enzymatic reaction, cell tsika, uye molecular biology maitiro.

Sero tsika: HEPES sodium munyu inoshandiswa zvakanyanya sechinhu chinodzivirira musero tsika midhiya.Iko kugona kuchengetedza yakagadzikana pH kwakakosha pakukura kwakaringana uye kushanda kwemasero.HEPES inowanzofarirwa pane mamwe ma-buffering agents sezvo isingaratidzi shanduko huru mupH kana yaiswa mumhepo CO2.

Zvidzidzo zveEnzyme: HEPES sodium munyu inonyanya kubatsira muzvidzidzo zve enzymatic uko inogara uye inodzorwa pH nharaunda inodiwa.Iyo inodzivirira kushanduka kwakanyanya mupH panguva yekuita enzymatic, kuve nechokwadi chekuita kwakaringana enzyme.

Electrophoresis: HEPES sodium munyu inowanzoshandiswa sechinhu chinopisa mumhando dzakasiyana dze electrophoretic dzakadai se polyacrylamide gel electrophoresis (PEJI) uye agarose gel electrophoresis.Inobatsira kuchengetedza pH yebuffer, iyo yakakosha pakuparadzanisa uye kuongorora nucleic acids uye mapuroteni.

Biochemical assays: HEPES sodium munyu inowanzo shandiswa mune dzakasiyana siyana biochemical assays, kusanganisira enzyme assays, protein quantification assays, uye spectrophotometric assays.Inobatsira kuchengetedza yaidiwa pH renji inodiwa kune yakarurama uye inobereka mhedzisiro.

Kugadzirwa kwezvinodhaka: HEPES sodium munyu inoshandiswawo mukugadzirwa kwemishonga semushonga webuffering kudzikamisa kugadzirwa kwezvinodhaka uye kuchengetedza inodikanwa pH renji.