HEPES-Na CAS:75277-39-3 Mutengo wemugadziri

Mushonga wekugadzirisa: HEPES sodium salt inowanzo shandiswa semushonga wekudzivirira mukuyedza kwezvipenyu nemakemikari. Inobatsira kuchengetedza pH yakagadzikana, kunyanya muhuwandu hwezvinhu zvipenyu (pH 7.2-7.6). Kugona kwayo kuchengetedza kunoita kuti ive yakakosha mukuchengetedza mamiriro akakodzera ekuita kwakasiyana-siyana kwema enzyme, tsika dzemasero, uye matekiniki e biology.

Kukura kwemasero: HEPES sodium salt inoshandiswa zvakanyanya senzira yekudzivirira masero kubva mukukura kwemasero. Kukwanisa kuchengetedza pH yakagadzikana kwakakosha pakukura kwakakodzera uye kurarama kwemasero. HEPES inowanzo sarudzwa pane dzimwe nzira dzekudzivirira masero sezvo isingaratidze shanduko huru mupH kana yasangana neCO2 yemhepo.

Zvidzidzo zveEnzyme: Munyu we sodium weHEPES unobatsira zvikuru muzvidzidzo zveEnzymatic uko kunodiwa pH inogara iripo uye inodzorwa. Inodzivirira kuchinja kukuru kwepH panguva yemaitiro eEnzymatic, zvichiita kuti enzyme ishande zvakanaka.

Electrophoresis: HEPES sodium salt inowanzo shandiswa senzira yekudzivirira masero mumhando dzakasiyana dze electrophoresis dzakadai se polyacrylamide gel electrophoresis (PAGE) uye agarose gel electrophoresis. Inobatsira kuchengetedza pH ye buffer, iyo inokosha pakuparadzanisa nekuongorora nucleic acids nemapuroteni.

Kuyedzwa kwemakemikari emuviri: Munyu we sodium weHEPES unoshandiswa kakawanda mukuyedzwa kwemakemikari akasiyana-siyana, kusanganisira kuyedzwa kwe enzyme, kuyedzwa kweprotein quantification, uye kuyedzwa kwe spectrophotometric. Inobatsira kuchengetedza pH inodiwa kuti pave nemhedzisiro chaiyo uye inodzokororwa.

Kugadzirwa kwemushonga: HEPES sodium salt inoshandiswawo mukugadzirwa kwemishonga senzira yekudzivirira kugadzikana kwemushonga uye kuchengetedza pH yaunoda.