Iodoacetamide CAS:144-48-9 Mutengo wemugadziri

Kugadziriswa kweCysteine: Iodoacetamide inopindirana nemapoka efree thiol mumaresidues ecysteine, zvichiita kuti pave nekubatana kwakabatana necysteine ​​​​sulfur. Kugadziriswa uku kunobatsira mukuongorora mapuroteni nekudzivirira maresidues ecysteine ​​uye kudzivirira maresidues asina kufanira kana oxidation.

Kugaya Mapuroteni: Iodoacetamide inowanzoshandiswa mukugaya mapuroteni pakuongorora mass spectrometry. Inoshanda pamwe chete nema cysteine ​​residues isati yagaya enzyme, ichidzivirira kuumbwa kwe disulfide bond isingadiwe uye ichichengetedza protein iri mumamiriro ayo asina kusimba.

Zvidzidzo zveProteomics: Iodoacetamide inoshandiswa mumatanho ekunyora mazita anosanganisira mass spectrometry-based proteomics. Kuburikidza nekuita kwayo necysteine ​​residues, inogonesa kusarudza mazita uye kuziva mapeptide ane cysteine.

Zvidzidzo zveMagadzirirwo eMapuroteni: Iodoacetamide inoshandiswa muzvidzidzo zvemagadzirirwo emakemikari kugadzirisa maresidues ecysteine ​​uye kudzidza mashandiro awo uye kubatanidzwa kwawo mukuchinja kwemapuroteni, kupeta, uye kuchinja kwechimiro. Inogona zvakare kubatsira mukuyedza kwekubatanidza mapuroteni kuti iongorore kudyidzana kwemapuroteni nemapuroteni.

Kugadzirwa kwePeptides Dzakanyorwa: Iodoacetamide inogona kushandiswa kugadzira mapeptide akanyorwa nekushandura maresidues ecysteine ​​nemakemikari akanyorwa neisotopically, akadai sedeuterium kana carbon-13. Mapeptide aya akanyorwa anoshandiswa mumabasa akasiyana-siyana ekutsvagisa, kusanganisira protein quantification uye dynamics studies.