N-Ethylmaleimide CAS:128-53-0 Mutengo wemugadziri

N-Ethylmaleimide (NEM) chinhu chidiki chinoshandiswa zvakanyanya muongororo ye biochemistry uye molecular biology. Inoshanda sechinhu chinodzivisa mapoka eprotein sulfhydryl (thiol) nekuchinja uye kuvharira mashandiro avo zvisingadzoreki. NEM inoshanda zvakanyanya nemapoka esulfhydryl, akadai seaya anowanikwa mu amino acid cysteine, uye inogona kuita nemapoka esulfhydryl akasununguka uye ari mumapuroteni. Izvi zvinoita kuti NEM ive chishandiso chinobatsira pakudzidza mashandiro emapuroteni, kudyidzana kwemapuroteni nemapuroteni, uye mashandiro ema enzyme. Hunhu hwayo hwekudzivirira hwakashandiswa mumabasa akasiyana-siyana, kusanganisira proteomics, enzymology, structural biology, uye kuwanikwa kwemishonga.

Odha kana Kumbira Mutengo

Ruzivo rweChigadzirwa

Matagi eChigadzirwa

Kushandiswa uye Mhedzisiro

Kugadziriswa kweMapuroteni: NEM inoshandura zvisingachinjike maresidues ecysteine mumapuroteni nekugadzira covalent adduct. Kugadziriswa uku kunogona kukanganisa mashandiro eprotein, chimiro, uye kudyidzana. Kubatanidzwa kweNEM nemapoka esulfhydryl kunoita kuti ive chishandiso chakakosha chekudzidza mashandiro eprotein uye maitiro anoenderana nethiol.

Kudziviswa kwemaEnzymes: MaEnzymes mazhinji anovimba nemapoka ethiol kuti aite basa rawo rekugadzirisa zvinhu. Nekuita zvinoenderana nemapoka ethiol, NEM inogona kudzivirira maEnzymes chaiwo nenzira yakasarudzika. Izvi zvave zvichishandiswa zvakanyanya kudzidza kinetics yeenzyme, kunzwisisa mashandiro emaEnzymatic, uye kudzivirira enzymes.

Zvidzidzo zveKushanda pamwe chete kweMapuroteni neMapuroteni: NEM inowanzoshandiswa kuongorora kufambidzana kwemapuroteni neprotein. Nekuvharira zvakasara zvecysteine, NEM inodzivirira kuumbwa kwezvisungo zve disulfide pakati pemapuroteni anobatanidzwa, nokudaro zvichikanganisa kufambidzana kwawo. Izvi zvinobvumira vaongorori kudzidza basa rema cysteine chaiwo mukufambidzana kwemapuroteni neprotein.

Zvidzidzo zveRedox Regulation: NEM inoshandiswa mukudzidza nzira dzeredox regulation mumapuroteni. Nekugadzirisa reactive cysteines, NEM inogona kudzikamisa mapuroteni chaiwo uye kudzivirira oxidation isingadiwe kana kudzikiswa kwemaresidues ecysteine. Izvi zvinobatsira mukunzwisisa basa re redox signaling uye redox-sensitive regulatory pathways.

Zvidzidzo zveCrosslinking: NEM inogona kushandiswa semushonga wekubatanidza mapuroteni kuti isimbise mapuroteni kana kudyidzana kwemapuroteni nemapuroteni. Nekugadzirisa ma cysteine residues, NEM inogona kugadzira covalent bonds pakati pemapuroteni anowirirana, zvichibvumira vaongorori kuongorora mapuroteni complexes nemaumbirwo awo. Iyi nzira inobatsira zvikuru muzvidzidzo zve structural biology uye proteomics.

Kuwanikwa Kwemishonga: Nekuda kwekugona kwayo kushandira pamwe chete nezvinosara zvecysteine, NEM yakashandiswa mukutsvaga mishonga. Inogona kushandiswa kuona nekuratidza zvinotarisirwa nemishonga kana kudzidza kuti mishonga yacho ingave yakakosha sei kune mapuroteni ane cysteine.

Kurongedza Chigadzirwa:

Mamwe Mashoko:

Kuumbwa	C6H7NO2
Kuyedza	99%
Chitarisiko	upfu chena hwekristaro
Nhamba yeCAS	128-53-0
Kurongedza	Diki uye rakakura
Hupenyu hweSherufu	makore maviri
Kuchengetera	Chengeta munzvimbo inotonhorera uye yakaoma
Chitupa	ISO.