NBD chloride CAS: 10199-89-0

NBD chloride, inozivikanwawo se7-chloro-4-nitrobenzo-2-oxa-1,3-diazole, idhayi re fluorescent rinowanzoshandiswa mukushandiswa kwemakemikari nehupenyu. Iri muboka re nitrobenzoxadiazole (NBD) derivatives, iyo inoratidza hunhu hwakasimba hwe fluorescence.

NBD chloride inoratidzwa nekukwanisa kwayo kuumba ma covalent bonds nema nucleophiles, akadai se primary amines, thiols, uye hydroxyl groups. Hunhu uhwu hunoibvumira kushandiswa se labeling agent ye biomolecules, kusanganisira mapuroteni, ma peptides, nucleic acids, uye mamwe mamorekuru madiki.

Kana NBD chloride ikapindirana ne nucleophile, inochinja kuita chimwe chinhu, zvichikonzera kuumbwa kwechinhu chakasimba chine label yeNBD. Chigadzirwa ichi chine label chinoratidza kupenya kwakanyanya, zvichiita kuti chibatsire pakuongorora nekushandisa nzira dzakasiyana siyana dzekufungidzira.

Kubuda kwechiedza chefluorescence kwemamorekuru ane label yeNBD kunogona kusimudzirwa nechiedza cheultraviolet (UV) pa470 nm, uye chiedza chinobuda chinowanzoonekwa pa530 nm. Kureba kwechiedza ichi kunowanikwa munzvimbo yakasvibira yespectrum inoonekwa, uko kukanganiswa kwekuona kubva kumasampuli ehupenyu kwakaderera.

Odha kana Kumbira Mutengo

Ruzivo rweChigadzirwa

Matagi eChigadzirwa

Kushandiswa uye Mhedzisiro

Kunyora ma "covalent labeling": NBD chloride inogona kuumba ma "covalent bonds" ane ma "nucleophilic groups" mu "biomolecules", akadai se "primary amines", "thiols", uye "hydroxyl groups". Izvi zvinobvumira kunyora ma "biomolecules" aya zvakananga kuti zvionekwe uye zvionekwe.

Kuwedzerwa kweFluorescence: Kana NBD chloride ikapindirana ne nucleophiles, inoumba chigadzirwa chine fluorescent NBD-labeled. Chigadzirwa ichi chine fluorescence chinoratidza fluorescence emission yakasimba, zvichiita kuti chibatsire pakuona uye kutevedza mamorekuru ane zvakanyorwa.

Kunzwa uye kujeka: Kubuda kwechiedza kwezvigadzirwa zvine label yeNBD kunonyanya kujeka, zvichiita kuti pave nekuonekwa kwemamorekuru mashoma ane label. Uyezve, kuumbwa kwe covalent bond pakati peNBD chloride ne nucleophiles kunoita kuti pave nekujeka, sezvo zvichiita kuti dhayi ibatane zvakanaka uye zvakananga ne biomolecule.

Kunyora ma "biomolecule": NBD chloride inogona kushandiswa kunyora ma "biomolecule" akasiyana-siyana, anosanganisira mapuroteni, mapeptide, nucleic acids, nemamwe mamorekuru madiki. Izvi zvinoita kuti vaongorori vakwanise kuongorora nzvimbo, simba, uye kudyidzana kwema "biomolecule" aya muma "biological systems".

Kuongororwa kwechiedza cheFluorescence: Ma biomolecule ane label yeNBD anogona kuonekwa uchishandisa fluorescence microscopy. Iyi nzira inobvumira kuona mamorekuru ane label padanho remasero kana pasi pemasero, zvichipa ruzivo rwakakosha nezvekupararira kwawo uye maitiro awo.

Kuyerera kwemasero: Ma biomolecule ane label yeNBD anogona kuongororwa uye kuverengwa uchishandisa flow cytometry. Iyi nzira inobvumira kuongororwa kwepamusoro-soro kwemasero kana zvidimbu zvine label, zvichipa ruzivo nezvemazinga ekutaura kana hunhu hwepamusoro hwema biomolecule ane label.

Kunyora mapuroteni uye kutevedza: NBD chloride inogona kushandiswa kunyora maamino acid residues kana mapoka anoshanda mumapuroteni. Izvi zvinogonesa vaongorori kudzidza chimiro chepuroteni, kupeta, mafambiro, uye kudyidzana munguva chaiyo.

Kurongedza Chigadzirwa:

Mamwe Mashoko:

Kuumbwa	C6H2ClN3O3
Kuyedza	99%
Chitarisiko	Upfu hweyero
Nhamba yeCAS	10199-89-0
Kurongedza	Diki uye rakakura
Hupenyu hweSherufu	makore maviri
Kuchengetera	Chengeta munzvimbo inotonhorera uye yakaoma
Chitupa	ISO.