Nzira yekuziva sei kuti yakanyanya kunaka sei?
Kune inducer IPTG (isopropyl-beta-d-thiogalactoside), huwandu hwakawanda hwechinhu, hunowedzera kunaka. Hunhu hwakanaka hunoenderana nemamiriro chaiwo ekuyedza uye mhedzisiro inodiwa yekudzikisa.
Kazhinji, huwandu hweIPTG hunoshandiswa pakati pe0.1-1 mM. Kuwanda kwakaderera kunogona kuderedza migumisiro yakaipa pakukura kwemasero uye kunogona kuderedza chepfu inouraya masero nekuda kwekuwedzera kwemapuroteni anotarisirwa. Kuwanda kwakawanda kunogona kukonzera mutoro wakawandisa wemetabolism yemasero, zvichikanganisa kukura kwemasero uye kushanda zvakanaka kwekutaura.
Nzira yekuona huwandu hwakakodzera inogona kuve yekuongorora huwandu hweproteni inotarwa nekuita bvunzo dzeIPTG dzekutanga pahuwandu hwakasiyana. Miedzo midiki yekumera inogona kuitwa uchishandisa huwandu hwakasiyana hweIPTG (semuenzaniso 0.1 mM, 0.5 mM, 1 mM, nezvimwewo) uye mhedzisiro yekutaura pahuwandu hwakasiyana inogona kuongororwa nekuona huwandu hweproteni inotarwa (semuenzaniso Western blot kana fluorescence detection). Zvichienderana nemhedzisiro yekuyedza, huwandu hwakanakisisa hwekutaura hwakasarudzwa sehuwandu hwakanakisisa.
Pamusoro pezvo, unogona zvakare kutarisa mabhuku akakodzera kana ruzivo rwemamwe marabhoritari kuti unzwisise huwandu hwehuwandu hweIPTG hunoshandiswa zvakanyanya pasi pemamiriro akafanana ekuyedza, wobva wagadzirisa uye wagadzirisa zvinoenderana nezvinodiwa zvekuyedza.
Zvakakosha kuziva kuti huwandu hwakakodzera hwemasero hunogona kusiyana zvichienderana nemaitiro akasiyana ekutaura, mapuroteni anotarisirwa, uye mamiriro ekuyedza, saka zvakanaka kugadzirisa mamiriro acho ezvinhu zvichienderana nemamiriro ezvinhu.
Nguva yekutumira: Gunyana-28-2023