Kune magamba mazhinji asina kurumbidzwa akatipoteredza, anoita seakajairika, asi kutaura chokwadi anopa zvakawanda chinyararire kwatiri. Proteinase K ndiyo "gamba risingazivikanwe" muindasitiri yekuongorora mamorekuru, kunyangwe ichienzaniswa ne "guru uye rine simba" muindasitiri, proteinase K haina musoro zvekuti takambokanganwa kukosha kwayo. Nekutanga kwedenda idzva rekorona, kudiwa kweproteinase K kwakawedzera, uye kuwanikwa kweproteinase munyika nekune dzimwe nyika kuri kure nekushandiswa, uye munhu wese akangoerekana aona kuti proteinase K yakakosha zvikuru.
Chii chinoshandiswa neproteinase K?
Proteinase K iprotease ine proteolytic enzyme activity uye inogona kuchengetedza kushanda munzvimbo dzakasiyana-siyana (pH (4-12.5), high-salt buffer, high tembiricha ye70°C, nezvimwewo). Pamusoro pezvo, kushanda kweproteinase K hakudzivisike neSDS, urea, EDTA, guanidine hydrochloride, guanidine isothiocyanate, nezvimwewo, uye huwandu hwakati hwesipo hunogonawo kuwedzera kushanda kweproteinase K. Mukurapa (hutachiona nehutachiona hwehutachiona), chikafu (kunyorovesa nyama), dehwe (kunyorovesa bvudzi), kugadzira waini (kujekesa doro), kugadzirira amino acid (minhenga yakaora), nucleic acid extraction, in situ hybridization, nezvimwewo, proteinase K Kune mashandisirwo. Kushandiswa kunonyanya kushandiswa ndeye nucleic acid extraction.
Proteinase K inogona kuita enzymolyze mhando dzese dzemapuroteni ari musampuli, kusanganisira ma histones akabatana zvakasimba ne nucleic acids, kuitira kuti nucleic acids dzikwanise kuburitswa kubva musampuli dzoburitswa muchidimbu, zvichiita kuti danho rinotevera rekubvisa nekucheneswa rive nyore. Pakuona viral nucleic acid, proteinase K chimwe chezvinhu zvakakosha mu virus sampling solution. Proteinase K inogona kupwanya nekumisa coat protein yevirus, iyo yakachengeteka panguva yekutakura nekuona; pamusoro pezvo, proteinase K inogonawo kuderedza RNase inodzivirira kuparara kweviral RNA uye inobatsira kuona nucleic acid.
Mukurumbira wenguva pfupi weproteinase K
Kungave kuri mumunda wekutsvaga kwesainzi kana mumunda weIVD, kuburitswa kwe nucleic acid ndiyo nzira iri nyore, saka proteinase K yagara iri chinhu chakakosha. Zvisinei, kare, proteinase K yaisazivikanwa zvakanyanya kupfuura basa rayo. Chikamu chikuru cheizvi chaive chekuti hukama hwekuwanikwa nekudiwa kweproteinase K hwaive hwakagadzikana. Vanhu vashoma vangafunga kuti kuwanikwa kweproteinase K kwaizova dambudziko.
Nekutanga kwedenda idzva rekorona, kudiwa kwekuyedzwa kwe nucleic acid kwakawedzera. Kusvika pakupera kwaChikumi 2020, China yakapedza bvunzo itsva dzekorona dzinosvika mamiriyoni makumi mapfumbamwe, uye nhamba iyi inototyisa pasi rose. Mukuyedza kwekubvisa nucleic acid, huwandu hweproteinase K hunoshanda hunenge 50-200 μg/mL. Kazhinji, zvinotora anenge 100 μg yeproteinase K kuti ibudise sampuro ye nucleic acid. Mukushandiswa chaiko, kuti kuwedzere kushanda kwekubvisa nucleic acid, kazhinji Proteinase K inoshandiswa nehuwandu hwakawedzerwa. Kuonekwa kweNucleic acid ye coronavirus itsva kwakaunza kudiwa kukuru kweproteinase K. Kuwanda kwekutanga kwekugovera nekudiwa kweproteinase K kwakakurumidza kuputswa, uye proteinase K yakava chinhu chakakosha chekudzivirira denda husiku humwe.
Matambudziko mukugadzirwa kweproteinase K
Kunyangwe nekukura kwedenda iri, kukosha kweproteinase K kwave kuchikosheswa nevanhu, zvinosuwisa kuti nekuda kwekusakwana kweproteinase K, makambani mashoma emuno akabatanidzwa mukugadzirwa kweproteinase K. Vanhu pavanoda kutanga kugadzirwa kweproteinase K Munguva yekugadzira, zvakaonekwa kuti proteinase K ipuroteni yakakosha zvikuru. Zvinonetsa zvikuru kuwedzera kugona kwekugadzira proteinase K munguva pfupi.
Kugadzirwa kukuru kweproteinase K kunosangana nematambudziko anotevera.
1. Kusataura zvakanaka
Proteinase K inogona kukanganisa mapuroteni akawanda uye kukonzera chepfu yakanyanya kumasero emuviri. Nokudaro, huwandu hweproteinase K hunowanzova hwakaderera. Kuongororwa kwemasisitimu emuviri uye mhando dzinoburitsa proteinase K zvakanyanya kunoda nguva yakareba.
2. Zvisaririra zve pigments uye nucleic acids
Kuvirisa kukuru kunounza huwandu hwakawanda hwepigment uye nucleic acid residues. Zvakaoma kubvisa tsvina iyi nenzira iri nyore yekuchenesa, uye kuchenesa kwakaoma kunowedzera mutengo uye kunoderedza mwero wekudzoreredza.
3. Kusagadzikana
Proteinase K haina kugadzikana zvakakwana, inogona kuzvigadzira enzyme, uye zvakaoma kuichengeta yakadzikama pa37°C kwenguva yakareba isina chinhu chinodzivirira.
4. Zviri nyore kupinza
Pakugadzirira upfu hwakaomeswa nechando hweproteinase K, kuitira kuti uve nechokwadi chekuti huwandu hwakasimba hweproteinase K muupfu hwakaomeswa nechando hwakakura, zvinodikanwa kuwedzera chinhu chinodzivirira chakaomeswa nechando pahuwandu hwakawanda, asi kana huwandu hweproteinase K hwasvika 20mg/mL zvichikwira, zviri nyore. Kuunganidzwa kunoita kuti pave nechando, izvo zvinounza matambudziko makuru pakuomesa kweproteinase K nehukuru hwakasimba.
5. Kudyara mari yakawanda
Proteinase K ine protease yakawanda uye inogona hydrolyze mamwe mapuroteni murabhoritari. Saka, proteinase K inoda nzvimbo dzakagadzirwa, michina, uye vashandi vekutsvaga nekugadzira.
Mhinduro yeXD BIOCHEM yeproteinase K
XD BIOCHEM ine puratifomu yakakura yekuburitsa mapuroteni uye yekuchenesa, uye ine ruzivo rwakakura mukubudisa nekucheneswa kwemapuroteni akasanganiswa uye kugadzirisa maitiro ekugadzira. Kuburikidza nekukasira kwechikwata chekutsvagisa nekugadzira, maitiro makuru ekugadzira proteinase K akakundwa. Kubuda kwemwedzi wega wega kweupfu hwakaomeswa nechando kunodarika 30 KG. Chigadzirwa ichi chine mashandiro akagadzikana, chiitiko chakanyanya che enzyme, uye hachina cytochrome uye nucleic acid residues. Tinokugamuchirai kuti mubate XD BIOCHEM Tora package yekuyedza (E-mail:sales@xdbiochem.comNhare: +86 513 81163739).
Mhinduro dzehunyanzvi dzeXD BIOCHEM dzinosanganisira
Uchishandisa kubatanidza plasmid ine makopi akawanda, mhando dzematauriro epamusoro dzine huwandu hwe8g/L dzinosarudzwa, izvo zvinokunda dambudziko rehuwandu hwematauriro eproteinase K.
Kuburikidza nekugadzirwa kwenzira yekuchenesa ine matanho akawanda, maresidues eproteinase K emugadziri we cytochrome ne nucleic acid akawanikwa kubva muproteinase K akabviswa zvinobudirira pasi pehuwandu hwakajairika.
Kuburikidza nekuongorora kwakawanda kwemhando dzeprotective buffer, buffer inogona kuchengetedza proteinase K zvakanaka pa37°C yakasarudzwa.
Kuchengetedza proteinase K kunopedza dambudziko rekuti proteinase K iri nyore kuunganidza nekuibvisa kana yakawandisa, uye ndiko kunoita kuti proteinase K iome nechando.
Muenzaniso weXD BIOCHEM proteinase K
XD BIOCHEM proteinase K bvunzo yekugadzikana: hapana shanduko huru mukushanda mushure memazuva makumi masere patembiricha yemumba
XD BIOCHEM proteinase K test stability: hapana shanduko huru mukushanda mushure memazuva makumi masere patembiricha yemumba.
Kuenzanisa kwemhedzisiro yekubviswa kwe nucleic acid yeXD BIOCHEM proteinase K nezvimwe zvigadzirwa zvinokwikwidzana. Mukuita kwekubviswa kwe nucleic acid, XD BIOCHEM uye competitive proteinase K zvinoshandiswa zvakateerana. Kubudirira kwekubviswa kwe XD BIOCHEM proteinase K kwakakwira uye kukosha kweCt kwegene rakatarwa kwakaderera.
Nguva yekutumira: Zvita-31-2021