ONPG CAS:369-07-3 Mutengo Wemugadziri

Mhedzisiro yeONPG se substrate inofanirwa kutsemurwa ne enzyme β-galactosidase, zvichikonzera kuburitswa kwechigadzirwa cheyero, o-nitrophenol.Uku kushanduka kwemavara kunogona kuyerwa spectrophotometrically, zvichibvumira kuwanda kwechiitiko che β-galactosidase.Kushandiswa kweONPG kunonyanya kuongorora kuratidzwa kwegene mu molecular biology uye microbiology tsvakurudzo.Inowanzoshandiswa kuyera basa re β-galactosidase semutori wenhau wezvidzidzo zvejeni, kunyanya mumabhakitiriya akadai seE. coli.The lacZ gene, iyo encodes β-galactosidase, inowanzoshandiswa sechiratidzo chekuongorora gene expression, sezvo kutaura kwaro kunogona kukonzerwa nemamiriro ezvinhu chaiwo kana kudzorwa nevanosimudzira. gene expression nekuyera basa re β-galactosidase.Ichi chiyedzo chinoshandiswa zvakanyanya mumashandisirwo akasiyana siyana, sekudzidza basa rekusimudzira, gene regulation, uye protein-protein kudyidzana.Pamusoro pezvo, inogona kushandiswa kuona enzyme kinetics uye kuongorora mhedzisiro yekuchinja kana marapirwo pane enzyme basa.