ONPG CAS:369-07-3 Mutengo wemugadziri

Mhedzisiro yeONPG se substrate inofanira kuchekwa ne enzyme β-galactosidase, zvichikonzera kuburitswa kwechinhu cheyero, o-nitrophenol. Kuchinja kweruvara uku kunogona kuyerwa ne spectrophotometric, zvichibvumira kuverengwa kwebasa re β-galactosidase. Kushandiswa kweONPG kunonyanya kuongororwa kwekutaura kwemajini muongororo ye molecular biology uye microbiology. Inowanzoshandiswa kuyera basa re β-galactosidase semutori wenhau wezvidzidzo zvekutaura kwemajini, kunyanya mumabhakitiriya akadai seE. coli. Jini re lacZ, iro rinonyora β-galactosidase, rinowanzo shandiswa sechiratidzo chekuongorora kutaura kwemajini, sezvo kutaura kwaro kunogona kukonzerwa nemamiriro ezvinhu chaiwo kana kudzorwa nevanokurudzira. Kuedza kweONPG kunopa nzira iri nyore uye yakavimbika yekuongorora huwandu hwekutaura kwemajini nekuyera basa re β-galactosidase. Kuedza uku kunoshandiswa zvakanyanya mukushandiswa kwakasiyana-siyana, senge kudzidza basa rekukurudzira, kutonga kwemajini, uye kudyidzana kwemapuroteni nemapuroteni. Pamusoro pezvo, inogona kushandiswa kuona kinetics yeenzyme uye kuongorora mhedzisiro yekuchinja kwemasero kana kurapwa pabasa reenzyme.