Nitrotetrazolium Blue Chloride CAS:298-83-9

Nitrotetrazolium Blue Chloride (NBT) chiratidzo che redox chinowanzoshandiswa mukuongorora kwehupenyu nemakemikari. Ihupfu hweyero hwakachenuruka hunoshanduka kuita bhuruu kana hwakaderedzwa, zvichiita kuti hubatsire pakuona kuvapo kwema enzymes uye mashandiro emetabolism.

NBT inobatana nemagetsi anotakura maerekitironi nemaenzyme akadai se dehydrogenases, ayo anobatanidzwa mumabasa akasiyana-siyana emasero. Kana NBT ikaderedzwa nema enzyme aya, inoumba bhuruu re formazan precipitate, zvichibvumira kuonekwa kwemaziso kana spectrophotometric.

Reagent iyi inowanzo shandiswa mukuyedza kwakadai seNBT reduction test, uko inoshandiswa kuongorora mashandiro ekufema kwemasero ekudzivirira muviri. Inogona zvakare kushandiswa kudzidza mabasa ema enzyme uye nzira dzemetabolism mukutsvaga kune chekuita nekushushikana kwe oxidative, kugara zvakanaka kwemasero, uye kusiyana kwemasero.

NBT yakawana mashandisirwo muzvikamu zvakasiyana-siyana, zvinosanganisira microbiology, immunology, uye biology yemasero. Inoshandiswa zvakasiyana-siyana, yakagadzikana, uye iri nyore kushandisa, zvichiita kuti ive sarudzo inozivikanwa kune akawanda mapuroteni ekuyedza.

Odha kana Kumbira Mutengo

Ruzivo rweChigadzirwa

Matagi eChigadzirwa

Kushandiswa uye Mhedzisiro

Mhedzisiro huru yeNBT kuumbwa kweblue formazan precipitate kana ikaderedzwa nemamwe ma enzymes. Kuchinja kwemavara uku kunobvumira kuona kana kuona mashandiro ema enzyme.

NBT ine mashandisirwo akasiyana-siyana mukutsvaga nekuongorora chirwere. Heano mamwe emashandisirwo ayo makuru:

Kuyedzwa kwemaitiro ema enzyme: NBT inogona kushandiswa kuyera mashandiro ema dehydrogenases, ayo anobatanidzwa mumabasa akadai sekufema kwemasero uye metabolism. Nekutarisa kudzikiswa kweNBT kuita formazan, vaongorori vanogona kuongorora mashandiro ema enzymes aya.

Kuongororwa kwebasa remasero ekudzivirira muviri: NBT inowanzo shandiswa mukuyedza kweNBT kuderedza mashandiro emasero ekudzivirira muviri, kunyanya ma phagocytes. Muyedzo uyu unoyera kugona kwemasero aya kugadzira mhando dzeokisijeni dzinoita basa, izvo zvinogona kuderedza NBT uye kugadzira bhuruu.

Ongororo ye microbiology: NBT inoshandiswa mu microbiology kudzidza metabolism ye microbial uye kuongorora mashandiro ema enzymes chaiwo. Semuenzaniso, yakashandiswa kuona bacterial nitrate reductases kana formazan-forming bacteria.

Zvidzidzo zvekuchengetedza masero: Kuderedzwa kweNBT kunobvumira vaongorori kuongorora mashandiro emetabolism uye kugona kwemasero kurarama. Nekuyera kusimba kwechigadzirwa cheblue formazan, zvinokwanisika kuona huwandu hwemasero anoshanda mumuenzaniso wakapihwa.

Muenzaniso weChigadzirwa

Kurongedza Chigadzirwa:

Mamwe Mashoko:

Kuumbwa	C40H30ClN10O6+
Kuyedza	99%
Chitarisiko	Upfu hweyero
Nhamba yeCAS	298-83-9
Kurongedza	Diki uye rakakura
Hupenyu hweSherufu	makore maviri
Kuchengetera	Chengeta munzvimbo inotonhorera uye yakaoma
Chitupa	ISO.