P-NITROPHENYL BETA-D-LACTOPYRANOSIDE CAS:4419-94-7

Kuonekwa kwebeta-galactosidase chiitiko: PNPG inowanzoshandiswa mukuyera kuyera chiitiko che beta-galactosidase, enzyme inokonzeresa iyo hydrolysis yelactose kuita glucose uye galactose.Iyo hydrolysis yePNPG ne beta-galactosidase inoburitsa p-nitrophenol (pNP) molecule, iyo inogona kuonekwa spectrophotometrically nekuda kweyero ruvara.

Kuongororwa kwe enzyme inhibitors uye activators: PNPG inogona kushandiswa mukukwirira-kuburikidza kuongorora kuona makomisheni anogadzirisa beta-galactosidase chiitiko.Nekuyera chiyero chePNPG hydrolysis pamberi peakasiyana bvunzo makomisheni, vaongorori vanogona kuona inhibitors inoderedza kuita kwe enzyme kana activator inowedzera basa re enzyme.

Kudzidza kwe enzyme kinetics: Iyo hydrolysis yePNPG ne beta-galactosidase inotevera Michaelis-Menten kinetics, ichibvumira vaongorori kuti vatarise zvakakosha enzyme paramita senge yakanyanya reaction velocity (Vmax) uye Michaelis anogara ari (Km).Ruzivo urwu runobatsira mukunzwisisa iyo enzyme's substrate affinity uye catalytic performance.

Molecular biology application: Beta-galactosidase, iyo inotsemura PNPG, inowanzoshandiswa semutori wenhau gene mune molecular biology.Iyo PNPG substrate inowanzo shandiswa kuona uye kuona mataurirwo emutori wenhau gene, ichipa nzira iri nyore uye inonzwisisika yekuongorora mataurirwo ejeni mumasisitimu akasiyana ekuyedza.