Phenylgalactoside CAS:2818-58-8

Mhedzisiro pakuita kwe enzyme: Phenylgalactoside inowanzoshandiswa kuyera basa reiyo enzyme β-galactosidase.Kana phenylgalactoside iine hydrolyzed ne β-galactosidase, inoburitsa p-nitrophenol.Iko kuunganidzwa kwep-nitrophenol kunogona kuyerwa kwehuwandu, kupa ruzivo rwekuita kwe β-galactosidase.Mhedzisiro iyi inosungirirwa mumashandisirwo akadai seye enzyme assays uye yekuongorora masisitimu.

Gene expression analysis: Phenylgalactoside inowanzo shandiswa se substrate mune molecular biology kuedza kudzidza gene expression.Iro lacZ gene, iro rinoisa β-galactosidase, rinowanzo kusanganiswa nehurongwa hwekutevedzana kwemamwe majini ekufarira.Kutaura kwejini re lacZ uye hydrolysis ye phenylgalactoside ne β-galactosidase inogona kuratidza maitiro ekutaura uye chiyero chejini rinotarirwa riri kudzidzwa.

Kuongorora masisitimu: Phenylgalactoside chinhu chakakosha chekuongorora masisitimu anoshandisa β-galactosidase chiitiko.Mumwe muenzaniso unozivikanwa zvikuru ndeye bhuruu-chena yekuongorora nzira, iyo inoshandiswa kuona recombinant kana shandurudzo maseru mumamolecular biology kuyedza.Makoroni akabudirira kutora DNA recombinant kana kuti akawana genetic recombination acharatidza β-galactosidase, zvichiita kuti hydrolysis ye phenylgalactoside uye kuumbwa kweruvara rwebhuruu.

Kucheneswa kweprotein: Mune zvimwe zviitiko, phenylgalactoside inogona kushandiswa se ligand ye affinity chromatography kuchenesa mapuroteni anonyatso sunga kana anoitwa ne β-galactosidase.Protein yekufarira inogona kunge iine affinity tag kana fusion tag ine β-galactosidase-binding domain.Nekupfuudza musanganiswa weprotein kuburikidza nekoramu ine immobilized phenylgalactoside, puroteni inodiwa inogona kuchengetwa zvakasarudzwa uye yozoburitswa.