4-Methylumbelliferyl-beta-D-glucopyranoside CAS:18997-57-4

Mhedzisiro ye4-Methylumbelliferyl-beta-D-glucopyranoside (MUG) ndeyekushanda sechikamu cheenzyme beta-glucosidase. Iyi enzyme inocheka chisungo cheglucosidic cheMUG, zvichikonzera kuburitswa kwe4-Methylumbelliferone (4-MU). Kushandiswa kweMUG kunonyanya kuitwa mumunda we microbiology, kunyanya pakuona nekuona mabhakitiriya anogadzira beta-glucosidase. MUG inowanzo shandiswa pakuona Escherichia coli (E. coli) mumvura nemuenzaniso wechikafu. E. coli ine enzyme beta-glucosidase, iyo inogona hydrolyze MUG uye kugadzira chiratidzo che fluorescent pamberi pechiedza che ultraviolet (UV). Hunhu hwe fluorescent hwe4-MU hunoita kuti zvive nyore kuona uye kuverenga. Kana MUG substrate yaiswa hydrolyze, 4-MU inogadzirwa inoburitsa blue fluorescence, zvichiita kuti zvikwanise kuzivikanwa mabhakitiriya ane beta-glucosidase activity. Nzira iyi inowanzo shandiswa mukutarisa nharaunda uye kuyedza kuchengetedzeka kwechikafu, sezvo ichipa nzira inokurumidza uye inonzwisisika yekuona kusvibiswa kwebhakitiriya. Pamusoro pekushandiswa kwayo mu microbiology, MUG inogona zvakare kushandiswa kudzidza mashandiro uye kudziviswa kwe beta-glucosidase mukutsvagisa kwe biochemistry uye molecular biology. Kupenya kwayo kunogonesa kuyerwa kwe enzyme kinetics uye inogona kushandiswa kuongorora zvingangoita zvinodzivisa kana zvinokonzeresa beta-glucosidase activity. Kazhinji, MUG imusanganiswa unoshanda zvakasiyana-siyana unoshandiswa zvakanyanya muminda ye microbiology, biochemistry, uye molecular biology pakuona mashandiro e beta-glucosidase uye kuzivikanwa kwebhakitiriya inogadzira iyi enzyme.