Proteinase K ye tritirachium album CAS:39450-01-6

Proteinase K ienzyme inotorwa kubva kufungus inonzi Tritirachium album. I serine protease ine hunyanzvi hwakasiyana-siyana, zvichireva kuti inogona kupatsanura ma peptide bonds mumapuroteni akasiyana-siyana. Proteinase K inozivikanwa nekugona kwayo kuputsa mapuroteni zvinobudirira, zvichiita kuti ive chishandiso chinokosha mukushandiswa kwakasiyana-siyana.

Imwe yenzira huru dzekushandisa Proteinase K ndeyekutsvaga mamorekuru ebiology. Inonyanya kushandiswa pakubvisa mapuroteni eDNA neRNA kuti ibvise mapuroteni anogona kukanganisa kuongororwa kwemasero. Proteinase K inobatsira mukubvisa mapuroteni emasero uye kuderedza mapuroteni emasero, zvichibvumira kuparadzaniswa kwe nucleic acids dzemhando yepamusoro.

Proteinase K inoshandiswawo zvakanyanya mukuongorora mapuroteni uye zvidzidzo zvehunhu. Inogona kushandiswa mukugaya mapuroteni, zvichibvumira kugadzirwa kwezvidimbu zvepeptide zvinogona kuongororwa nenzira dzakadai semass spectrometry. Pamusoro pezvo, Proteinase K inobatsira pakuongorora mapuroteni uye kuona nzvimbo yemaamino acids mukati me polypeptide chain.

Imwe nzira yakakosha yeProteinase K ndeyekubvisa kusvibiswa pamusoro. Inowanzoshandiswa mumarabhoritari eMolecular biology kubvisa nucleic acids, mapuroteni, nezvimwe zvinosvibisa kubva mumidziyo yemarabhoritari nenzvimbo dzayo. Proteinase K yakasimba uye inoshanda mumamiriro akasiyana-siyana, kusanganisira tembiricha yakakwira uye pH mazinga akasiyana.